ArticlePloS one2017
Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 126 citations in OpenAlex.
- The Biofilm Plight in Healthcare: Orchestration and Control by Lactiplantibacillus plantarum.Probiotics and antimicrobial proteins · 2026Review
- Experiences and theory reveal a decrease in antimicrobial blue light killing efficiency as biofilm grows in a Pseudomonas aeruginosa model.Scientific reports · 2026Article
- Article
- Elastic Deformation of Cellulose/Lignin-Based Anode for Rejuvenating Aged Mix-Cultured Electroactive Biofilms.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Ultrasound-activated cilia for biofilm control in indwelling medical devices.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The role of fluid friction in streamer formation and biofilm growth.NPJ biofilms and microbiomes · 2025Article
- Shear stress controls prokaryotic and eukaryotic biofilm communities together with EPS and metabolomic expression in a semi-controlled coastal environment in the NW Mediterranean Sea.Environmental microbiome · 2024Article
- Effects of fluid shear stress on oral biofilm formation and composition and the transcriptional response of Streptococcus gordonii.Molecular oral microbiology · 2024Article
- Effect of shear rate on earlyBiofilm · 2024Article
- Biofilm formation ofHeliyon · 2024Article
- Fluid flow drives phenotypic heterogeneity in bacterial growth and adhesion on surfaces.Nature communications · 2024Article
- Microfluidic Platform with Precisely Controlled Hydrodynamic Parameters and Integrated Features for Generation of Microvortices to Accurately Form and Monitor Biofilms in Flow.ACS biomaterials science & engineering · 2024Article
- Biofilm inhibition/eradication: exploring strategies and confronting challenges in combatting biofilm.Archives of microbiology · 2024Review
- Balancing water conservation and health: do water-saving showerheads impact the microbes we breathe in during showering?Frontiers in microbiomes · 2024Article
- Community-forming traits play role in effective colonization of plant-growth-promoting bacteria and improved plant growth.Frontiers in plant science · 2024Article
- A Millifluidic Chamber for Controlled Shear Stress Testing: Application to Microbial Cultures.Annals of biomedical engineering · 2023Article
- A standard 96-well based high throughput microfluidic perfusion biofilm reactor for in situ optical analysis.Biomedical microdevices · 2023Article
- An Enzybiotic Cocktail Effectively Disrupts Preformed Dual Biofilm ofPharmaceuticals (Basel, Switzerland) · 2023Article
- The interplay between bacterial biofilms, encrustation, and wall shear stress in ureteral stents: a review across scales.Frontiers in urology · 2023Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial communities attached to surfaces under fluid flow represent a widespread lifestyle of the microbial world. Through shear stress generation and molecular transport regulation, hydrodynamics conveys effects that are very different by nature but strongly coupled. To decipher the influence of these levers on bacterial biofilms immersed in moving fluids, we quantitatively and simultaneously investigated physicochemical and biological properties of the biofilm. We designed a millifluidic setup allowing to control hydrodynamic conditions and to monitor biofilm development in real time using microscope imaging. We also conducted a transcriptomic analysis to detect a potential physiological response to hydrodynamics. We discovered that a threshold value of shear stress determined biofilm settlement, with sub-piconewton forces sufficient to prevent biofilm initiation. As a consequence, distinct hydrodynamic conditions, which set spatial distribution of shear stress, promoted distinct colonization patterns with consequences on the growth mode. However, no direct impact of mechanical forces on biofilm growth rate was observed. Consistently, no mechanosensing gene emerged from our differential transcriptomic analysis comparing distinct hydrodynamic conditions. Instead, we found that hydrodynamic molecular transport crucially impacts biofilm growth by controlling oxygen availability. Our results shed light on biofilm response to hydrodynamics and open new avenues to achieve informed design of fluidic setups for investigating, engineering or fighting adherent communities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.