Evidence map›Paper›PMID 28403156›Full record

ArticlePloS one2017

Direct fluorescence detection of VirE2 secretion by Agrobacterium tumefaciens.

Noga Yaakov, Yoav Barak, Idan Pereman, Peter J Christie, Michael Elbaum

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Noga YaakovDept of Materials and Interfaces, Weizmann Institute of Science, Rehovot, Israel.
Yoav BarakChemical Research Support Dept, Weizmann Institute of Science, Rehovot, Israel.
Idan PeremanDept of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.
Peter J ChristieDepartment of Microbiology and Molecular Genetics, UT-Houston Medical School, Houston, Texas, United States of America.
Michael ElbaumDept of Materials and Interfaces, Weizmann Institute of Science, Rehovot, Israel.
Weizmann Institute of Science · IL

Funding

TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPER01GM048746 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI CHRISTIE, PETER J. · 1998 to 2018
$6.9M
NIGMS NIH HHS R01 GM048746
6 · The paper itself

Abstract

VirE2 is a ssDNA binding protein essential for virulence in Agrobacterium tumefaciens. A tetracysteine mutant (VirE2-TC) was prepared for in vitro and in vivo fluorescence imaging based on the ReAsH reagent. VirE2-TC was found to be biochemically active as it binds both ssDNA and the acidic secretion chaperone VirE1. It was also biologically functional in complementing virE2 null strains transforming Arabidopsis thaliana roots and Nicotiana tabacum leaves. In vitro experiments demonstrated a two-color fluorescent complex using VirE2-TC/ReAsH and Alexa Fluor 488 labeled ssDNA. In vivo, fluorescent VirE2-TC/ReAsH was detected in bacteria and in plant cells at time frames relevant to transformation.

Indexed as

Agrobacterium tumefaciensArabidopsisBacterial ProteinsDNA-Binding ProteinsDNA, Single-StrandedMutationNicotianaOptical ImagingPlant TumorsProtein BindingVirulence FactorsBacterial ProteinsDNA-Binding ProteinsDNA, Single-StrandedVirulence Factors

Identifiers

PMID28403156
PMCPMC5389803
OpenAlexW2606938190

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.