ArticleNucleic acids research2017
A non-canonical multisubunit RNA polymerase encoded by the AR9 phage recognizes the template strand of its uracil-containing promoters.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Phage host range: determinants, dynamics and applications.Nature reviews. Microbiology · 2026Review
- The biology of jumbo phages.Nature communications · 2026Review
- Structure of the bacteriophage PhiKZ non-virion RNA polymerase bound to a p119L open promoter analogue.IUCrJ · 2026Article
- A ribosome-interacting jumbophage protein associates with the phage nucleus to facilitate efficient propagation.PLoS pathogens · 2025Article
- Structure of the Bacteriophage PhiKZ Non-virion RNA Polymerase Transcribing from its Promoter p119L.Journal of molecular biology · 2024Article
- Tail-tape-fused virion and non-virion RNA polymerases of a thermophilic virus with an extremely long tail.Nature communications · 2024Article
- Genomic and proteomic characterization of vB_SauM-UFV_DC4, a novel Staphylococcus jumbo phage.Applied microbiology and biotechnology · 2023Article
- The Dynamics of Synthesis and Localization of Jumbo Phage RNA Polymerases inside Infected Cells.Viruses · 2023Article
- Phage-Encoded Sigma Factors Alter Bacterial Dormancy.mSphere · 2022Article
- Flagellotropic Bacteriophages: Opportunities and Challenges for Antimicrobial Applications.International journal of molecular sciences · 2022Review
- Structural basis of template strand deoxyuridine promoter recognition by a viral RNA polymerase.Nature communications · 2022Article
- Computational models in the service of X-ray and cryo-electron microscopy structure determination.Proteins · 2021Article
- Comparative Genomics of Three Novel Jumbo Bacteriophages Infecting Staphylococcus aureus.Journal of virology · 2021Article
- Structure of the bacteriophage PhiKZ non-virion RNA polymerase.Nucleic acids research · 2021Article
- Article
- Multisubunit RNA Polymerases of Jumbo Bacteriophages.Viruses · 2020Review
- Global Proteomic Profiling ofJournal of virology · 2019Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
AR9 is a giant Bacillus subtilis phage whose uracil-containing double-stranded DNA genome encodes distant homologs of β and β' subunits of bacterial RNA polymerase (RNAP). The products of these genes are thought to assemble into two non-canonical multisubunit RNAPs - a virion RNAP (vRNAP) that is injected into the host along with phage DNA to transcribe early phage genes, and a non-virion RNAP (nvRNAP), which is synthesized during the infection and transcribes late phage genes. We purified the AR9 nvRNAP from infected B. subtilis cells and characterized its transcription activity in vitro. The AR9 nvRNAP requires uracils rather than thymines at specific conserved positions of late viral promoters. Uniquely, the nvRNAP recognizes the template strand of its promoters and is capable of specific initiation of transcription from both double- and single-stranded DNA. While the AR9 nvRNAP does not contain homologs of bacterial RNAP α subunits, it contains, in addition to the β and β'-like subunits, a phage protein gp226. The AR9 nvRNAP lacking gp226 is catalytically active but unable to bind to promoter DNA. Thus, gp226 is required for promoter recognition by the AR9 nvRNAP and may represent a new group of transcription initiation factors.
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