ArticleBiotechnology and bioengineering2017
A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae.
Article in Biotechnology and bioengineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Accelerating natural product discovery, characterization and engineering by biofoundries.Natural product reports · 2026Review
- Protocol for connector-enabled multigene pathway assembly using Golden Gate Assembly and yeast recombination.STAR protocols · 2026Article
- Review
- A Novel Biosynthetic Strategy for Ginsenoside Ro: Construction of a Metabolically EngineeredInternational journal of molecular sciences · 2024Article
- Reconstruction of a robust bacterial replication module.Nucleic acids research · 2024Article
- Sustainable production of natural products using synthetic biology: Ginsenosides.Journal of ginseng research · 2024Review
- Genome mining unveils a class of ribosomal peptides with two amino termini.Nature communications · 2023Article
- Genome mining unveils a class of ribosomal peptides with two amino termini.bioRxiv : the preprint server for biology · 2023Article
- Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli.Biochemical Society transactions · 2022Review
- A scalable platform to discover antimicrobials of ribosomal origin.Nature communications · 2022Article
- MicroED in natural product and small molecule research.Natural product reports · 2021Review
- Recent Advances in Strategies for the Cloning of Natural Product Biosynthetic Gene Clusters.Frontiers in bioengineering and biotechnology · 2021Review
- Discovery and Characterization of a Class IV Lanthipeptide with a Nonoverlapping Ring Pattern.ACS chemical biology · 2020Article
- Emerging molecular biology tools and strategies for engineering natural product biosynthesis.Metabolic engineering communications · 2020Review
- Control of nitrogen fixation in bacteria that associate with cereals.Nature microbiology · 2020Article
- Recent Advances in the Metabolic Engineering of Yeasts for Ginsenoside Biosynthesis.Frontiers in bioengineering and biotechnology · 2020Review
- Targeting Bacterial Genomes for Natural Product Discovery.Trends in pharmacological sciences · 2020Review
- Changing Form and Function through Carotenoids and Synthetic Biology.Plant physiology · 2019Article
- Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering.Nucleic acids research · 2019Article
- Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli.ACS chemical biology · 2018Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Pathway refactoring serves as an invaluable synthetic biology tool for natural product discovery, characterization, and engineering. However, the complicated and laborious molecular biology techniques largely hinder its application in natural product research, especially in a high-throughput manner. Here we report a plug-and-play pathway refactoring workflow for high-throughput, flexible pathway construction, and expression in both Escherichia coli and Saccharomyces cerevisiae. Biosynthetic genes were firstly cloned into pre-assembled helper plasmids with promoters and terminators, resulting in a series of expression cassettes. These expression cassettes were further assembled using Golden Gate reaction to generate fully refactored pathways. The inclusion of spacer plasmids in this system would not only increase the flexibility for refactoring pathways with different number of genes, but also facilitate gene deletion and replacement. As proof of concept, a total of 96 pathways for combinatorial carotenoid biosynthesis were built successfully. This workflow should be generally applicable to different classes of natural products produced by various organisms. Biotechnol. Bioeng. 2017;114: 1847-1854. © 2017 Wiley Periodicals, Inc.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.