ArticlePlant methods2017
A rapid and efficient method for uniform gene expression using the barley stripe mosaic virus.
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Modulation of the wheat transcriptome by TaZFP13D under well-watered and drought conditions.Plant molecular biology · 2024Article
- Article
- Article
- A New Method for Rapid Subcellular Localization and Gene Function Analysis in Cotton Based on Barley Stripe Mosaic Virus.Plants (Basel, Switzerland) · 2022Article
- Nanoparticles for protein delivery in planta.Current opinion in plant biology · 2021Review
- A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants.Frontiers in plant science · 2021Review
- An EfficientFrontiers in plant science · 2021Article
- The barley stripe mosaic virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance.BMC plant biology · 2020Article
- A Hedychium coronarium short chain alcohol dehydrogenase is a player in allo-ocimene biosynthesis.Plant molecular biology · 2019Article
- Gene silencing approaches through virus-based vectors: speeding up functional genomics in monocots.Plant molecular biology · 2019Review
- Review
- An efficient and improved method for virus-induced gene silencing in sorghum.BMC plant biology · 2018Article
- A New Barley Stripe Mosaic Virus Allows Large Protein Overexpression for Rapid Function Analysis.Plant physiology · 2018Article
- Systemic Infection ofBioMed research international · 2018Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe barley stripe mosaic virus (BSMV) has become a popular vector to study gene function in cereals. However, studies have been limited to gene silencing in leaves of barley or wheat. In addition, the method produces high variability between different leaves and plants. To overcome these limitations, we explored the potential of modifying the inoculation protocol for BSMV gene overexpression. An improved light, oxygen or voltage-sensing (iLOV) domain-based fluorescent protein was used as a reporter of gene expression to monitor the infection and spread of BSMV. Tobacco (
resultsCompared to the leaf abrasion method, the seed imbibition method resulted in a high and uniform detection of iLOV in both roots and leaves of different wheat cultivars and other monocot and dicot species within 7 days after germination. The progression of viral infection via the imbibition method as measured by the expression of iLOV was more stable in different organs and tissues and is transmissible to the next generation.
conclusionOur results show that BSMV can be used as a vector for the expression of small genes such as
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