ArticleScientific reports2017
High Throughput Analysis of Golgi Structure by Imaging Flow Cytometry.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- SIGMA: SIgnaling via Golgi Morphology Analysis for pathway-state classification.bioRxiv : the preprint server for biology · 2026Article
- VPS13B, gene responsible for Cohen syndrome, regulates gingival epithelial barrier function via intracellular trafficking of coxsackievirus and adenovirus receptor.Scientific reports · 2026Article
- High-content phenotyping reveals Golgi dynamics and their role in cell cycle regulation.The Journal of cell biology · 2026Article
- Imaging flow cytometry-based cellular screening elucidates pathophysiology in individuals with Variants of Uncertain Significance.Genome medicine · 2025Article
- Imaging Flow Cytometry: Development, Present Applications, and Future Challenges.Methods and protocols · 2024Review
- Streptococcus pneumoniae disrupts the structure of the golgi apparatus and subsequent epithelial cytokine response in an HCell communication and signaling : CCS · 2023Article
- A circuit for secretion-coupled cellular autonomy in multicellular eukaryotic cells.Molecular systems biology · 2023Article
- Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Applying imaging flow cytometry and immunofluorescence in studying the dynamic Golgi structure in cultured cells.STAR protocols · 2022Article
- Review
- Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.iScience · 2021Article
- Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages.Frontiers in cellular and infection microbiology · 2021Article
- Imaging-Based Reporter Systems to Define CVB-Induced Membrane Remodeling in Living Cells.Viruses · 2020Article
- Mutant p53 induces Golgi tubulo-vesiculation driving a prometastatic secretome.Nature communications · 2020Article
- Kinetics of Mimivirus Infection Stages Quantified Using Image Flow Cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- High-throughput imaging of zebrafish embryos using a linear-CCD-based flow imaging system.Biomedical optics express · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Golgi apparatus is a dynamic organelle, which regulates the vesicular trafficking. While cellular trafficking requires active changes of the Golgi membranes, these are not accompanied by changes in the general Golgi's structure. However, cellular processes such as mitosis, apoptosis and migration require fragmentation of the Golgi complex. Currently, these changes are most commonly studied by basic immunofluorescence and quantified by manual and subjective classification of the Golgi structure in 100-500 stained cells. Several other high-throughput methods exist as well, but those are either complicated or do not provide enough morphological information. Therefore, a simple and informative high content methodology should be beneficial for the study of Golgi architecture. Here we describe the use of high-throughput imaging flow cytometry for quantification of Golgi fragmentation, which provides a simple way to analyze the changes in an automated, quantitative and non-biased manner. Furthermore, it provides a rapid and accurate way to analyze more than 50,000 cells per sample. Our results demonstrate that this method is robust and statistically powerful, thus, providing a much-needed analytical tool for future studies on Golgi dynamics, and can be adapted to other experimental systems.
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