Evidence map›Paper›PMID 28400398›Full record

ReviewCirculation research2017

Multi-Imaging Method to Assay the Contractile Mechanical Output of Micropatterned Human iPSC-Derived Cardiac Myocytes.

Alexandre J S Ribeiro, Olivier Schwab, Mohammad A Mandegar, Yen-Sin Ang, Bruce R Conklin, Deepak Srivastava, Beth L Pruitt

Open access · bronzeAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Circulation research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed, 1 pooled it
5.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 1 synthesis or guideline pooled it, 127 citations in OpenAlex.

  1. Pooled it
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  15. Incomplete-penetrant hypertrophic cardiomyopathyProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  16. Review
  17. Field Guide to Traction Force Microscopy.Cellular and molecular bioengineering · 2024
    Review
  18. Dynamic control of contractile resistance to iPSC-derived micro-heart muscle arrays.Journal of biomedical materials research. Part A · 2024
    Article
  19. Review
  20. Article

10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Alexandre J S RibeiroFrom the Department of Mechanical Engineering (A.J.S.R., O.S., B.L.P.), Department of Molecular and Cellular Physiology (by courtesy) (B.L.P.), Department of Bioengineering (by courtesy) (B.L.P.), and Stanford Cardiovascular Institute (A.J.S.R., B.L.P.), Stanford University, CA; Gladstone Institute of Cardiovascular Disease, San Francisco, CA (A.J.S.R., M.A.M., Y.-S.A., B.R.C., D.S.); Roddenberry Stem Cell Center at Gladstone, San Francisco, CA (Y.-S.A., D.S.); Departments of Pediatrics and Biochemistry & Biophysics (D.S.), Department of Cellular and Molecular Pharmacology (B.R.C.), California Institute for Quantitative Biosciences, QB3 (B.R.C.), and Department of Medicine and Cellular and Molecular Pharmacology (B.R.C.), University of California, San Francisco.
Olivier SchwabFrom the Department of Mechanical Engineering (A.J.S.R., O.S., B.L.P.), Department of Molecular and Cellular Physiology (by courtesy) (B.L.P.), Department of Bioengineering (by courtesy) (B.L.P.), and Stanford Cardiovascular Institute (A.J.S.R., B.L.P.), Stanford University, CA; Gladstone Institute of Cardiovascular Disease, San Francisco, CA (A.J.S.R., M.A.M., Y.-S.A., B.R.C., D.S.); Roddenberry Stem Cell Center at Gladstone, San Francisco, CA (Y.-S.A., D.S.); Departments of Pediatrics and Biochemistry & Biophysics (D.S.), Department of Cellular and Molecular Pharmacology (B.R.C.), California Institute for Quantitative Biosciences, QB3 (B.R.C.), and Department of Medicine and Cellular and Molecular Pharmacology (B.R.C.), University of California, San Francisco.
Mohammad A MandegarFrom the Department of Mechanical Engineering (A.J.S.R., O.S., B.L.P.), Department of Molecular and Cellular Physiology (by courtesy) (B.L.P.), Department of Bioengineering (by courtesy) (B.L.P.), and Stanford Cardiovascular Institute (A.J.S.R., B.L.P.), Stanford University, CA; Gladstone Institute of Cardiovascular Disease, San Francisco, CA (A.J.S.R., M.A.M., Y.-S.A., B.R.C., D.S.); Roddenberry Stem Cell Center at Gladstone, San Francisco, CA (Y.-S.A., D.S.); Departments of Pediatrics and Biochemistry & Biophysics (D.S.), Department of Cellular and Molecular Pharmacology (B.R.C.), California Institute for Quantitative Biosciences, QB3 (B.R.C.), and Department of Medicine and Cellular and Molecular Pharmacology (B.R.C.), University of California, San Francisco.
Yen-Sin AngFrom the Department of Mechanical Engineering (A.J.S.R., O.S., B.L.P.), Department of Molecular and Cellular Physiology (by courtesy) (B.L.P.), Department of Bioengineering (by courtesy) (B.L.P.), and Stanford Cardiovascular Institute (A.J.S.R., B.L.P.), Stanford University, CA; Gladstone Institute of Cardiovascular Disease, San Francisco, CA (A.J.S.R., M.A.M., Y.-S.A., B.R.C., D.S.); Roddenberry Stem Cell Center at Gladstone, San Francisco, CA (Y.-S.A., D.S.); Departments of Pediatrics and Biochemistry & Biophysics (D.S.), Department of Cellular and Molecular Pharmacology (B.R.C.), California Institute for Quantitative Biosciences, QB3 (B.R.C.), and Department of Medicine and Cellular and Molecular Pharmacology (B.R.C.), University of California, San Francisco.
Bruce R ConklinFrom the Department of Mechanical Engineering (A.J.S.R., O.S., B.L.P.), Department of Molecular and Cellular Physiology (by courtesy) (B.L.P.), Department of Bioengineering (by courtesy) (B.L.P.), and Stanford Cardiovascular Institute (A.J.S.R., B.L.P.), Stanford University, CA; Gladstone Institute of Cardiovascular Disease, San Francisco, CA (A.J.S.R., M.A.M., Y.-S.A., B.R.C., D.S.); Roddenberry Stem Cell Center at Gladstone, San Francisco, CA (Y.-S.A., D.S.); Departments of Pediatrics and Biochemistry & Biophysics (D.S.), Department of Cellular and Molecular Pharmacology (B.R.C.), California Institute for Quantitative Biosciences, QB3 (B.R.C.), and Department of Medicine and Cellular and Molecular Pharmacology (B.R.C.), University of California, San Francisco.
Deepak SrivastavaFrom the Department of Mechanical Engineering (A.J.S.R., O.S., B.L.P.), Department of Molecular and Cellular Physiology (by courtesy) (B.L.P.), Department of Bioengineering (by courtesy) (B.L.P.), and Stanford Cardiovascular Institute (A.J.S.R., B.L.P.), Stanford University, CA; Gladstone Institute of Cardiovascular Disease, San Francisco, CA (A.J.S.R., M.A.M., Y.-S.A., B.R.C., D.S.); Roddenberry Stem Cell Center at Gladstone, San Francisco, CA (Y.-S.A., D.S.); Departments of Pediatrics and Biochemistry & Biophysics (D.S.), Department of Cellular and Molecular Pharmacology (B.R.C.), California Institute for Quantitative Biosciences, QB3 (B.R.C.), and Department of Medicine and Cellular and Molecular Pharmacology (B.R.C.), University of California, San Francisco.
Beth L PruittFrom the Department of Mechanical Engineering (A.J.S.R., O.S., B.L.P.), Department of Molecular and Cellular Physiology (by courtesy) (B.L.P.), Department of Bioengineering (by courtesy) (B.L.P.), and Stanford Cardiovascular Institute (A.J.S.R., B.L.P.), Stanford University, CA; Gladstone Institute of Cardiovascular Disease, San Francisco, CA (A.J.S.R., M.A.M., Y.-S.A., B.R.C., D.S.); Roddenberry Stem Cell Center at Gladstone, San Francisco, CA (Y.-S.A., D.S.); Departments of Pediatrics and Biochemistry & Biophysics (D.S.), Department of Cellular and Molecular Pharmacology (B.R.C.), California Institute for Quantitative Biosciences, QB3 (B.R.C.), and Department of Medicine and Cellular and Molecular Pharmacology (B.R.C.), University of California, San Francisco. pruitt@stanford.edu.
Gladstone Institutes · US

Funding

Force Clamp Systems for Evaluation of MechanotransductionR01EB006745 · NIBIB · STANFORD UNIVERSITY · PI GOODMAN, MIRIAM B, PRUITT, BETH L · 2007 to 2014
$3.1M
Identifying Therapeutic Targets for RNA Splicing-Related CardiomyopathyR01HL130533 · NHLBI · J. DAVID GLADSTONE INSTITUTES · PI CONKLIN, BRUCE R · 2016 to 2019
$1.8M
Validating engineered hiPSC-derived cardiomyocytes as model cellsR21HL130993 · NHLBI · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI PRUITT, BETH L · 2016 to 2017
$434k
NHLBI NIH HHS R01 HL130533NHLBI NIH HHS R21 HL130993NIBIB NIH HHS R01 EB006745
6 · The paper itself

Abstract

rationaleDuring each beat, cardiac myocytes (CMs) generate the mechanical output necessary for heart function through contractile mechanisms that involve shortening of sarcomeres along myofibrils. Human-induced pluripotent stem cells (hiPSCs) can be differentiated into CMs (hiPSC-CMs) that model cardiac contractile mechanical output more robustly when micropatterned into physiological shapes. Quantifying the mechanical output of these cells enables us to assay cardiac activity in a dish.

objectiveWe sought to develop a computational platform that integrates analytic approaches to quantify the mechanical output of single micropatterned hiPSC-CMs from microscopy videos. METHODS AND

resultsWe micropatterned single hiPSC-CMs on deformable polyacrylamide substrates containing fluorescent microbeads. We acquired videos of single beating cells, of microbead displacement during contractions, and of fluorescently labeled myofibrils. These videos were independently analyzed to obtain parameters that capture the mechanical output of the imaged single cells. We also developed novel methods to quantify sarcomere length from videos of moving myofibrils and to analyze loss of synchronicity of beating in cells with contractile defects. We tested this computational platform by detecting variations in mechanical output induced by drugs and in cells expressing low levels of myosin-binding protein C.

conclusionsOur method can measure the cardiac function of single micropatterned hiPSC-CMs and determine contractile parameters that can be used to elucidate mechanisms that underlie variations in CM function. This platform will be amenable to future studies of the effects of mutations and drugs on cardiac function.

Indexed as

Cells, CulturedHumansInduced Pluripotent Stem CellsMultimodal ImagingMyocardial ContractionMyocytes, Cardiaccardiac myocytecontractilitysarcomere lengthsingle cellstem cell

Identifiers

PMID28400398
PMCPMC5491345
OpenAlexW2894495525

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.