Evidence map›Paper›PMID 28399915›Full record

ArticleBMC cancer2017

Tiamulin inhibits breast cancer growth and pulmonary metastasis by decreasing the activity of CD73.

Xu Yang, Shimin Pei, Huanan Wang, Yipeng Jin, Fang Yu, Bin Zhou, Hong Zhang, Di Zhang, Degui Lin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Xu YangThe Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Shimin PeiThe Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Huanan WangDepartment of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Yipeng JinThe Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Fang YuThe Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Bin ZhouThe Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Hong ZhangThe Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Di ZhangThe Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. dzhangdvm@cau.edu.cn.
Degui LinThe Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. csama@sina.com.ORCID 0000-0003-1326-9861
China Agricultural University · CNZhejiang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMetastasis is the leading cause of death in breast cancer patients. CD73, also known as ecto-5'-nucleotidase, plays a critical role in cancer development including metastasis. The existing researches indicate that overexpression of CD73 promotes growth and metastasis of breast cancer. Therefore, CD73 inhibitor can offer a promising treatment for breast cancer. Here, we determined whether tiamulin, which was found to inhibit CD73, was able to suppress breast cancer development and explored the related mechanisms.

methodsWe firstly measured the effect of tiamulin hydrogen fumarate (THF) on CD73 using high performance liquid chromatography (HPLC). Then, we investigated cell proliferation, migration and invasion in MDA-MB-231 human breast cancer cell line and 4 T1 mouse breast cancer cell line treated with THF by migration assay, invasion assay and activity assay. Besides, we examined the effect of THF on syngeneic mammary tumors of mice by immunohistochemistry.

resultsOur data demonstrated that THF inhibited CD73 by decreasing the activity instead of the expression of CD73. In vitro, THF inhibited the proliferation, migration and invasion of MDA-MB-231 and 4 T1 cells by suppressing CD73 activity. In vivo, animal experiments showed that THF treatment resulted in significant reduction in syngeneic tumor growth, microvascular density and lung metastasis rate.

conclusionsOur results indicate that THF inhibits growth and metastasis of breast cancer by blocking the activity of CD73, which may offer a promising treatment for breast cancer therapy.

Indexed as

5'-NucleotidaseAnimalsAnti-Bacterial AgentsApoptosisBreast NeoplasmsCell MovementCell ProliferationDiterpenesFemaleGene Expression Regulation, NeoplasticGPI-Linked ProteinsHumansLung NeoplasmsMiceMice, Inbred BALB CMice, Nude5'-NucleotidaseAnti-Bacterial AgentsDiterpenesGPI-Linked ProteinsNT5E protein, humantiamulinAngiogenesisBreast cancerCD73MetastasisThf

Identifiers

PMID28399915
PMCPMC5387263
OpenAlexW2607425056

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.