ArticleJournal of experimental botany2017
Immunogold scanning electron microscopy can reveal the polysaccharide architecture of xylem cell walls.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Combined TIRF and 3D Super-Resolution Microscopy for Nanoscopic Characterization of Adhesion Molecules on Microvilli.Analytical chemistry · 2026Article
- Stomata-targeted nanocarriers enhance plant defense against pathogen colonization.Nature communications · 2025Article
- Advanced imaging-enabled understanding of cell wall remodeling mechanisms mediating plant drought stress tolerance.Frontiers in plant science · 2025Review
- Establishment and application of highly efficient regeneration, genetic transformation and genome editing system for cucurbitacins biosynthesis in Hemsleya chinensis.BMC plant biology · 2024Article
- Xylem-dwelling pathogen unaffected by local xylem vessel network properties in grapevines (Vitis spp.).Annals of botany · 2024Article
- Physicochemical and antioxidant properties of pectin fromFrontiers in nutrition · 2024Article
- Physicochemical properties of tiger nut (Food chemistry: X · 2023Article
- Polysaccharides from South TunisianPlants (Basel, Switzerland) · 2023Article
- Structural variation and spatial polysaccharide profiling of intervessel pit membranes in grapevine.Annals of botany · 2022Article
- Ultrastructural histochemistry in biomedical research: Alive and kicking.European journal of histochemistry : EJH · 2018Article
- Infrared Nanospectroscopy Reveals the Chemical Nature of Pit Membranes in Water-Conducting Cells of the Plant Xylem.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunofluorescence microscopy (IFM) and immunogold transmission electron microscopy (TEM) are the two main techniques commonly used to detect polysaccharides in plant cell walls. Both are important in localizing cell wall polysaccharides, but both have major limitations, such as low resolution in IFM and restricted sample size for immunogold TEM. In this study, we have developed a robust technique that combines immunocytochemistry with scanning electron microscopy (SEM) to study cell wall polysaccharide architecture in xylem cells at high resolution over large areas of sample. Using multiple cell wall monoclonal antibodies (mAbs), this immunogold SEM technique reliably localized groups of hemicellulosic and pectic polysaccharides in the cell walls of five different xylem structures (vessel elements, fibers, axial and ray parenchyma cells, and tyloses). This demonstrates its important advantages over the other two methods for studying cell wall polysaccharide composition and distribution in these structures. In addition, it can show the three-dimensional distribution of a polysaccharide group in the vessel lateral wall and the polysaccharide components in the cell wall of developing tyloses. This technique, therefore, should be valuable for understanding the cell wall polysaccharide composition, architecture and functions of diverse cell types.
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