ArticleJournal of clinical laboratory analysis2018
Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV.
Article in Journal of clinical laboratory analysis, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Evaluation of a fully automatic nucleic acid amplification system for detecting four respiratory pathogens.Pediatric research · 2025Article
- Agreement of respiratory viruses' detection between nasopharyngeal swab and bronchoalveolar lavage in adults admitted for pneumonia: a retrospective study.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2023Article
- Snotwatch: an ecological analysis of the relationship between febrile seizures and respiratory virus activity.BMC pediatrics · 2022Article
- Treatment of Respiratory Viral Coinfections.Epidemiologia (Basel, Switzerland) · 2022Article
- Comparison of the AdvanSure RV Plus Real-Time RT-PCR and Real-Q RV II Detection Assays for Respiratory Viruses.Annals of laboratory medicine · 2021Article
- Comparison of the clinical characteristics and mortality of adults infected with human coronaviruses 229E and OC43.Scientific reports · 2021Article
- The prevalence of viral infections in children with cystic fibrosis in a tertiary care center in Saudi Arabia.International journal of pediatrics & adolescent medicine · 2020Article
- Evaluation of a commercial latex agglutination test for detecting rotavirus A and human adenovirus in children's stool specimens.Journal of clinical laboratory analysis · 2020Article
- Viral respiratory infections diagnosed by multiplex polymerase chain reaction in pediatric patients.Journal of medical virology · 2019Article
- Multiplex Platforms for the Identification of Respiratory Pathogens: Are They Useful in Pediatric Clinical Practice?Frontiers in cellular and infection microbiology · 2019Review
- Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV.Journal of clinical laboratory analysis · 2018Article
- The epidemiology and severity of respiratory viral infections in a tropical country: Ecuador, 2009-2016.Journal of infection and public healthArticle
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDue to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses.
methodsA total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays.
resultsOf the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%-98%, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94%-100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results.
conclusionsWe suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.
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