ArticleScientific reports2017
An optimized transit peptide for effective targeting of diverse foreign proteins into chloroplasts in rice.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Light-regulated dual-targeting of NUCLEAR CONTROL OF PEP ACTIVITY establishes photomorphogenesis via interorganellar communication.Plant physiology · 2025Article
- Expression of an antimicrobial peptide persulcatusin fused with calmodulin in rice cultured cells.Transgenic research · 2025Article
- Synthetic photorespiratory bypass improves rice productivity by enhancing photosynthesis and nitrogen uptake.The Plant cell · 2024Article
- Identification of a highly efficient chloroplast-targeting peptide for plastid engineering.PLoS biology · 2024Article
- Poaceae plants transfer cyclobutane pyrimidine dimer photolyase to chloroplasts for ultraviolet-B resistance.Plant physiology · 2024Article
- A synthetic light-inducible photorespiratory bypass enhances photosynthesis to improve rice growth and grain yield.Plant communications · 2023Article
- An optimized transformation protocol for Anthoceros agrestis and three more hornwort species.The Plant journal : for cell and molecular biology · 2023Article
- Cell-Penetrating Peptides for Use in Development of Transgenic Plants.Molecules (Basel, Switzerland) · 2023Review
- The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy.Plant biotechnology journal · 2022Article
- The nucleotide metabolome of germinating Arabidopsis thaliana seeds reveals a central role for thymidine phosphorylation in chloroplast development.The Plant cell · 2022Article
- Evaluation of biotransformation capacity of transplastomic plants and hairy roots of Nicotiana tabacum expressing human cytochrome P450 2D6.Transgenic research · 2022Article
- Developing a platform for production of the oxylipin KODA in plants.Journal of experimental botany · 2022Article
- Transit Peptides Often Require Downstream Unstructured Sequence for Efficient Chloroplast Import inFrontiers in plant science · 2022Article
- Biosynthetic approaches to efficient assimilation of COFrontiers in bioengineering and biotechnology · 2022Review
- Advances and prospects of rhodopsin-based optogenetics in plant research.Plant physiology · 2021Review
- Engineered Accumulation of Bicarbonate in Plant Chloroplasts: Known Knowns and Known Unknowns.Frontiers in plant science · 2021Review
- Use of synthetic biology tools to optimize the production of active nitrogenase Fe protein in chloroplasts of tobacco leaf cells.Plant biotechnology journal · 2020Article
- Two glyoxylate reductase isoforms are functionally redundant but required under high photorespiration conditions in rice.BMC plant biology · 2020Article
- Fluorescent reporters for functional analysis in rice leaves.Plant direct · 2020Article
- Cytological evidence of BSD2 functioning in both chloroplast division and dimorphic chloroplast formation in maize leaves.BMC plant biology · 2020Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Various chloroplast transit peptides (CTP) have been used to successfully target some foreign proteins into chloroplasts, but for other proteins these same CTPs have reduced localization efficiencies or fail completely. The underlying cause of the failures remains an open question, and more effective CTPs are needed. In this study, we initially observed that two E.coli enzymes, EcTSR and EcGCL, failed to be targeted into rice chloroplasts by the commonly-used rice rbcS transit peptide (rCTP) and were subsequently degraded. Further analyses revealed that the N-terminal unfolded region of cargo proteins is critical for their localization capability, and that a length of about 20 amino acids is required to attain the maximum localization efficiency. We considered that the unfolded region may alleviate the steric hindrance produced by the cargo protein, by functioning as a spacer to which cytosolic translocators can bind. Based on this inference, an optimized CTP, named RC2, was constructed. Analyses showed that RC2 can more effectively target diverse proteins, including EcTSR and EcGCL, into rice chloroplasts. Collectively, our results provide further insight into the mechanism of CTP-mediated chloroplastic localization, and more importantly, RC2 can be widely applied in future chloroplastic metabolic engineering, particularly for crop plants.
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