ArticleViruses2017
The Transmembrane Morphogenesis Protein gp1 of Filamentous Phages Contains Walker A and Walker B Motifs Essential for Phage Assembly.
Article in Viruses, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
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- Cryo-EM structure of a bacteriophage M13 mini variant.Nature communications · 2023Article
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- Characterisation of the Novel Filamentous Phage PMBT54 Infecting the Milk Spoilage BacteriaViruses · 2023Article
- Discovery of a novel filamentous prophage in the genome of theFrontiers in microbiology · 2023Article
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- Encapsulation and Delivery of Therapeutic Phages.Applied and environmental microbiology · 2021Review
- A Biological Inventory of Prophages inFrontiers in microbiology · 2020Article
- Secondary Bacterial Infections During Pulmonary Viral Disease: Phage Therapeutics as Alternatives to Antibiotics?Frontiers in microbiology · 2020Review
- The Pseudomonas aeruginosa accessory genome elements influence virulence towards Caenorhabditis elegans.Genome biology · 2019Article
- The SRP signal sequence of KdpD.Scientific reports · 2019Article
- 1st German Phage Symposium-Conference Report.Viruses · 2018Article
- Large conformational changes of a highly dynamic pre-protein binding domain in SecA.Communications biology · 2018Article
- Viruses of Microbes.Viruses · 2017Article
- Assisted and Unassisted Protein Insertion into Liposomes.Biophysical journal · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to lytic phages, filamentous phages are assembled in the inner membrane and secreted across the bacterial envelope without killing the host. For assembly and extrusion of the phage across the host cell wall, filamentous phages code for membrane-embedded morphogenesis proteins. In the outer membrane of Escherichia coli, the protein gp4 forms a pore-like structure, while gp1 and gp11 form a complex in the inner membrane of the host. By comparing sequences with other filamentous phages, we identified putative Walker A and B motifs in gp1 with a conserved lysine in the Walker A motif (K14), and a glutamic and aspartic acid in the Walker B motif (D88, E89). In this work we demonstrate that both, Walker A and Walker B, are essential for phage production. The crucial role of these key residues suggests that gp1 might be a molecular motor driving phage assembly. We further identified essential residues for the function of the assembly complex. Mutations in three out of six cysteine residues abolish phage production. Similarly, two out of six conserved glycine residues are crucial for gp1 function. We hypothesise that the residues represent molecular hinges allowing domain movement for nucleotide binding and phage assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.