Evidence map›Paper›PMID 28395914›Full record

ArticleTissue & cell2017

Structurally and functionally characterized in vitro model of rabbit vocal fold epithelium.

Masanobu Mizuta, Takashi Kurita, Emily E Kimball, Bernard Rousseau

Open access · hybridAbstract read
In one paragraph

Article in Tissue & cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Hydrogel-Supported, Engineered Model of Vocal Fold Epithelium.ACS biomaterials science & engineering · 2021
    Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Masanobu MizutaDepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
Takashi KuritaDepartment of Otolaryngology, Vanderbilt University Medical Center, 1215 21st Avenue South, Medical Center East, Nashville, TN 37232, USA.
Emily E KimballDepartment of Hearing and Speech Sciences, Vanderbilt University School of Medicine, 1215 21st Avenue South, Medical Center East, Nashville, TN 37232, USA.
Bernard RousseauDepartment of Otolaryngology, Vanderbilt University Medical Center, and Hearing and Speech Sciences and Mechanical Engineering, Vanderbilt University School of Medicine, Medical Center East, 1215 21st Avenue South, Nashville, TN 37232, USA. Electronic address: bernard.rousseau@vanderbilt.edu.
Vanderbilt University Medical Center · USKyoto University · JP

Funding

Tumor Immunology and Microenvironment Research ProgramP30CA068485 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI MATTHEW J TYSKA · 1995 to 2026
$172.8M
Vanderbilt Mouse Metabolic Physiology CenterU24DK059637 · NIDDK · VANDERBILT UNIVERSITY · PI WASSERMAN, DAVID H · 2001 to 2015
$14.9M
Pre-Clinical Testing of the Safety and Efficacy of Treatments for Voice DisordersR01DC015405 · NIDCD · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ROUSSEAU, BERNARD · 2016 to 2020
$2.8M
Molecular Pathophysiology of Acute PhonotraumaR01DC011338 · NIDCD · VANDERBILT UNIVERSITY · PI ROUSSEAU, BERNARD · 2011 to 2015
$1.9M
NCI NIH HHS P30 CA068485NIDCD NIH HHS R01 DC011338NIDCD NIH HHS R01 DC015405NIDDK NIH HHS U24 DK059637
6 · The paper itself

Abstract

In this paper, we describe a method for primary culture of a well differentiated electrically tight rabbit vocal fold epithelial cell multilayer and the measurement of transepithelial electrical resistance (TEER) for the evaluation of epithelial barrier function in vitro. Rabbit larynges were harvested and enzymatically treated to isolate vocal fold epithelial cells and to establish primary culture. Vocal fold epithelial cells were co-cultured with mitomycin C-treated feeder cells on collagen-coated plates. After 10-14 days in primary culture, cells were passaged and cultured until they achieved 70-90% confluence on collagen-coated plates. Epithelial cells were then passaged onto collagen-coated cell culture inserts using 4.5cm

Indexed as

Epithelial CellsModels, BiologicalRespiratory MucosaVocal CordsAnimalsCells, CulturedRabbitsEpithelial cellsIn vitroPrimary cultureTransepithelial resistanceVocal fold

Identifiers

PMID28395914
PMCPMC5467526
OpenAlexW2600895392

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.