Evidence map›Paper›PMID 28395731›Full record

ArticleeLife2017

A nanobody-based toolset to investigate the role of protein localization and dispersal in

Stefan Harmansa, Ilaria Alborelli, Dimitri Bieli, Emmanuel Caussinus, Markus Affolter

Open access · goldAbstract read
In one paragraph

Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.

0numbers the graph read from it
0cells of the map it votes in
65citing papers in PubMed
5.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

65 citing papers in PubMed, 113 citations in OpenAlex.

  1. Review
  2. Harnessing the Power ofCold Spring Harbor perspectives in biology · 2026
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5 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Stefan HarmansaGrowth and Development, Biozentrum, University of Basel, Basel, Switzerland.ORCID 0000-0001-6668-7608
Ilaria AlborelliGrowth and Development, Biozentrum, University of Basel, Basel, Switzerland.ORCID 0000-0001-7849-3958
Dimitri BieliGrowth and Development, Biozentrum, University of Basel, Basel, Switzerland.
Emmanuel CaussinusGrowth and Development, Biozentrum, University of Basel, Basel, Switzerland.
Markus AffolterGrowth and Development, Biozentrum, University of Basel, Basel, Switzerland.ORCID 0000-0002-5171-0016
University of Basel · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The role of protein localization along the apical-basal axis of polarized cells is difficult to investigate in vivo, partially due to lack of suitable tools. Here, we present the GrabFP system, a collection of four nanobody-based GFP-traps that localize to defined positions along the apical-basal axis. We show that the localization preference of the GrabFP traps can impose a novel localization on GFP-tagged target proteins and results in their controlled mislocalization. These new tools were used to mislocalize transmembrane and cytoplasmic GFP fusion proteins in the

Indexed as

Protein TransportAnimalsDrosophilaDrosophila ProteinsEntomologyGenes, ReporterGreen Fluorescent ProteinsMolecular BiologyRecombinant Fusion ProteinsSingle-Domain AntibodiesDrosophila ProteinsGreen Fluorescent ProteinsRecombinant Fusion ProteinsSingle-Domain Antibodiescell biologydevelopmental biologyD. melanogasterDppmorphogennanobodyprotein localizationstem cells

Identifiers

PMID28395731
PMCPMC5388529
OpenAlexW2597332893

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.