ArticleBiology of reproduction2017
Acidification of uterine epithelium during embryo implantation in mice.
Article in Biology of reproduction, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Blastocyst-Derived Lactic Acid Regulates Uterine Epithelial Receptivity and Stromal Decidualization via the HIF1α-HO-1-Heme Metabolic Axis.Antioxidants (Basel, Switzerland) · 2026Article
- Endometrial Epithelial Lactate Deficiency Drives CD8Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells.The Journal of reproduction and development · 2025Article
- Article
- H3K27me3 timely dictates uterine epithelial transcriptome remodeling and thus transformation essential for normal embryo implantation.Cell death and differentiation · 2024Article
- Attenuated retinoic acid signaling is among the early responses in mouse uterus approaching embryo attachment.Reproductive and developmental medicine · 2024Article
- Article
- Recurrent Implantation Failure: Bioinformatic Discovery of Biomarkers and Identification of Metabolic Subtypes.International journal of molecular sciences · 2023Article
- BMP/SMAD1/5 Signaling in the Endometrial Epithelium Is Essential for Receptivity and Early Pregnancy.Endocrinology · 2022Article
- A Role for Fructose Metabolism in Development of Sheep and Pig Conceptuses.Advances in experimental medicine and biology · 2022Article
- Transcriptome Comparison of Chorion-Attached and Non-chorion-attached Endometrium in Mid-gestation of Rabbit.Frontiers in veterinary science · 2022Article
- Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF.International journal of molecular sciences · 2021Article
- The therapeutic importance of acid-base balance.Biochemical pharmacology · 2021Review
- Functions of Lysosomes in Mammalian Female Reproductive System.Reproductive and developmental medicine · 2020Article
- ATP mediates the interaction between human blastocyst and endometrium.Cell proliferation · 2020Article
- Uterine Luminal Epithelium as the Transient Gateway for Embryo Implantation.Trends in endocrinology and metabolism: TEM · 2020Review
- Lactic Acid: A Novel Signaling Molecule in Early Pregnancy?Frontiers in immunology · 2020Review
- Nucleolar stress regulation of endometrial receptivity in mouse models and human cell lines.Cell death & disease · 2019Article
- Association of luteal cell degeneration and progesterone deficiency with lysosomal storage disorder mucolipidosis type IV in Mcoln1-/- mouse model†.Biology of reproduction · 2019Article
- Dietary exposure to mycotoxin zearalenone (ZEA) during post-implantation adversely affects placental development in mice.Reproductive toxicology (Elmsford, N.Y.) · 2019Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Uterine luminal epithelium (LE) is essential for establishing uterine receptivity. Previous microarray analysis revealed upregulation of Atp6v0d2 in gestation day 4.5 (D4.5) LE in mice. Realtime PCR showed upregulation of uterine Atp6v0d2 starting right before embryo attachment ∼D4.0. In situ hybridization demonstrated specific uterine localization of Atp6v0d2 in LE upon embryo implantation. Atp6v0d2 encodes one subunit for vacuolar-type H+-ATPase (V-ATPase), which regulates acidity of intracellular organelles and extracellular environment. LysoSensor Green DND-189 detected acidic signals in LE and glandular epithelium upon embryo implantation, correlating with Atp6v0d2 upregulation in early pregnant uterus. Atp6v0d2-/- females had significantly reduced implantation rate and marginally reduced delivery rate from first mating only, but comparable number of implantation sites and litter size compared to control and comparable fertility to control from subsequent matings, suggesting a nonessential role of Atp6v0d2 subunit in embryo implantation. Successful implantation in both control and Atp6v0d2-/- females was associated with uterine epithelial acidification. No significant compensatory upregulation of Atp6v0d1 mRNA was detected in D4.5 Atp6v0d2-/- uteri. To determine the role of V-ATPase instead of a single subunit in embryo implantation, a specific V-ATPase inhibitor bafilomycin A1 (2.5 μg/kg) was injected via uterine fat pad on D3 18:00 h. This treatment resulted in reduced uterine epithelial acidification, delayed implantation, and reduced number of implantation sites. It also suppressed oil-induced artificial decidualization. These data demonstrate uterine epithelial acidification as a novel phenomenon during embryo implantation and V-ATPase is involved in uterine epithelial acidification and uterine preparation for embryo implantation.
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Registered trials
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