ArticleNature methods2017
DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells.
Article in Nature methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 75 citations in OpenAlex.
- Actin disassembly triggers CNS myelin compaction and wrapping.bioRxiv : the preprint server for biology · 2026Article
- TGFβ induces an atypical EMT to evade immune mechanosurveillance in lung adenocarcinoma dormant metastasis.Nature cancer · 2026Article
- Elongation of the nascent avian foregut requires coordination of intrinsic and extrinsic cell behaviors.Developmental biology · 2025Article
- Article
- Application and measurement of tissue-scale tension in avian epithelia in vivo to study multiscale mechanics and inter-germ layer coupling.Development (Cambridge, England) · 2025Article
- A Chemogenetic Toolkit for Inducible, Cell Type-Specific Actin Disassembly.Small methods · 2025Article
- Using ALS to understand profilin 1's diverse roles in cellular physiology.Cytoskeleton (Hoboken, N.J.) · 2025Review
- Ectopic reconstitution of a spine-apparatus-like structure provides insight into mechanisms underlying its formation.Current biology : CB · 2025Article
- Mitochondria- and ER-associated actin are required for mitochondrial fusion.Nature communications · 2025Article
- The polar code for patterning: how polarity and the cytoskeleton orchestrate asymmetric cell division during plant development.Frontiers in cell and developmental biology · 2025Review
- Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistance.eLife · 2024Article
- Schwann cell-secreted PGECell · 2024Article
- Turn-on protein switches for controlling actin binding in cells.Nature communications · 2024Article
- Mitochondria- and ER-associated actin are required for mitochondrial fusion.bioRxiv : the preprint server for biology · 2024Article
- Optical Control of G-Actin with a Photoswitchable Latrunculin.Journal of the American Chemical Society · 2024Article
- SRF transcriptionally regulates the oligodendrocyte cytoskeleton during CNS myelination.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Oligodendrocyte calcium signaling promotes actin-dependent myelin sheath extension.Nature communications · 2024Article
- Characterization of the intracellular neurexin interactome by in vivo proximity ligation suggests its involvement in presynaptic actin assembly.PLoS biology · 2024Article
- Structure-function relationship of dynorphin B variants using naturally occurring amino acid substitutions.Frontiers in pharmacology · 2024Article
- Turn-On Protein Switches for Controlling Actin Binding in Cells.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 3 countries.
Funding
Abstract
The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.