ArticleScientific reports2017
Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 32 citations in OpenAlex.
- Repression of Polycomb Group Factor 1 Inhibits EMT Progression in Colon Cancers Cells via Wnt/β-Catenin and PI3K/Akt/mTOR Signaling Pathway.Digestive diseases and sciences · 2026Article
- Microglial PCGF1 alleviates neuroinflammation associated depressive behavior in adolescent mice.Molecular psychiatry · 2025Article
- Article
- Brachyury co-operates with polycomb protein RYBP to regulate gastrulation and axial elongationFrontiers in cell and developmental biology · 2024Article
- Inactivation of Tumor Suppressor CYLD Inhibits Fibroblast Reprogramming to Pluripotency.Cancers · 2023Article
- Pcgf1 gene disruption reveals primary involvement of epigenetic mechanism in neuronal subtype specification in the enteric nervous system.Development, growth & differentiation · 2023Article
- [PCGF1 is highly expressed in rectal adenocarcinoma and silencing PCGF1 inhibits proliferation of rectal adenocarcinoma cellsNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2022Article
- Critical Roles of Polycomb Repressive Complexes in Transcription and Cancer.International journal of molecular sciences · 2022Review
- The Role of Polycomb Proteins in Cell Lineage Commitment and Embryonic Development.Epigenomes · 2022Review
- Reversal of viral and epigenetic HLA class I repression in Merkel cell carcinoma.The Journal of clinical investigation · 2022Article
- Functional redundancy among Polycomb complexes in maintaining the pluripotent state of embryonic stem cells.Stem cell reports · 2022Article
- PCGF1 is a prognostic biomarker and correlates with tumor immunity in gliomas.Annals of translational medicine · 2022Article
- PCGF1 promotes epigenetic activation of stemness markers and colorectal cancer stem cell enrichment.Cell death & disease · 2021Article
- Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination.PLoS genetics · 2021Article
- Mammalian PRC1 Complexes: Compositional Complexity and Diverse Molecular Mechanisms.International journal of molecular sciences · 2020Review
- Functions of Polycomb Proteins on Active Targets.Epigenomes · 2020Review
- Chromatin regulation and dynamics in stem cells.Current topics in developmental biology · 2020Review
- Pcgf1 Regulates Early Neural Tube Development Through Histone Methylation in Zebrafish.Frontiers in cell and developmental biology · 2020Article
- Repression of PCGF1 Decreases the Proliferation of Glioblastoma Cells in Association with Inactivation of c-Myc Signaling Pathway.OncoTargets and therapy · 2020Article
- Epigenetic control of transcriptional regulation in pluripotency and early differentiation.Development (Cambridge, England) · 2019Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Polycomb repressive complex 1 (PRC1) is essential for fate decisions of embryonic stem (ES) cells. Emerging evidence suggests that six major variants of PRC1 complex, defined by the mutually exclusive presence of Pcgf subunit, regulate distinct biological processes, yet very little is known about the mechanism by which each version of PRC1 instructs and maintains cell fate. Here, we disrupted the Pcgf1, also known as Nspc1 and one of six Pcgf paralogs, in mouse ES cells by the CRISPR/Cas9 technology. We showed that although these mutant cells were viable and retained normal self-renewal, they displayed severe defects in differentiation in vitro. To gain a better understanding of the role of Pcgf1 in transcriptional control of differentiation, we analysed mRNA profiles from Pcgf1 deficient cells using RNA-seq. Interestingly, we found that Pcgf1 positively regulated expression of essential transcription factors involved in ectoderm and mesoderm differentiation, revealing an unexpected function of Pcgf1 in gene activation during ES cell lineage specification. Chromatin immunoprecipitation experiments demonstrated that Pcgf1 deletion caused a decrease in Ring1B and its associated H2AK119ub1 mark binding to target genes. Altogether, our results suggested an unexpected function of Pcgf1 in gene activation during ES cell maintenance.
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Registered trials
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