ArticleJournal of molecular histology2017
GATA4 regulates osteoblastic differentiation and bone remodeling via p38-mediated signaling.
Article in Journal of molecular histology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 29 citations in OpenAlex.
- IGF-1 Induces Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Promoting SOX4 via the MAPK/ERK Pathway.International journal of stem cells · 2024Article
- GATA4-activated lncRNA MALAT1 promotes osteogenic differentiation through inhibiting NEDD4-mediated RUNX1 degradation.Cell death discovery · 2023Article
- Article
- Viability, yield and expansion capability of feline MSCs obtained from subcutaneous and reproductive organ adipose depots.BMC veterinary research · 2021Article
- Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38.International journal of molecular sciences · 2020Article
- Dopamine D1 receptor-mediated activation of the ERK signaling pathway is involved in the osteogenic differentiation of bone mesenchymal stem cells.Stem cell research & therapy · 2020Article
- Elucidation on Predominant Pathways Involved in the Differentiation and Mineralization of Odontoblast-Like Cells by Selective Blockade of Mitogen-Activated Protein Kinases.BioMed research international · 2018Article
- Mechanical stress promotes matrix synthesis of mandibular condylar cartilage via the RKIP-ERK pathway.Journal of molecular histology · 2017Article
- Blockade of LGR4 inhibits proliferation and odonto/osteogenic differentiation of stem cells from apical papillae.Journal of molecular histology · 2017Article
- Differential expression of transforming growth factor-beta1, connective tissue growth factor, phosphorylated-SMAD2/3 and phosphorylated-ERK1/2 during mouse tooth development.Journal of molecular histology · 2017Article
- CTHRC1 promotes osteogenic differentiation of periodontal ligament stem cells by regulating TAZ.Journal of molecular histology · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
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Abstract
Osteoblasts play a major role in bone remodeling and are regulated by transcription factors. GATA4, a zinc finger transcription factor from the GATA family, has an unclear role in osteoblast differentiation. In this study, the role of GATA4 in osteoblast differentiation was studied both in vitro and in vivo by GATA4 knockdown. GATA4 expression increased during osteoblast differentiation. GATA4 knockdown in osteoblast precursor cells reduced alkaline phosphatase activity and decreased the formation of calcified nodule in an osteogenic-induced cell culture system. In vivo, micro-CT showed that local injection of lentivirus-delivered GATA4 shRNA caused reduced new bone formation during tooth movement. Histological analyses such as total collagen and Goldner's trichrome staining confirmed these results. In vivo immunohistochemical analysis showed reduced expression of osterix (OSX), osteopontin (OPN), and osteocalcin (OCN) in the shGATA4 group (P < 0.05). Consistently, both western blotting and quantitative reverse-transcription PCR proved that expression of osteogenesis-related genes, including OSX, OPN, and OCN, was significantly repressed in the shGATA4 group in vitro (P < 0.01). For further analysis of the pathways involved in this process, we examined the MAPK signaling pathway, and found knockdown of GATA4, downregulated p38 signaling pathways (P < 0.01). Collectively, these results imply GATA4 is a regulator of osteoblastic differentiation via the p38 signaling pathways.
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