ArticleChem2016
Enzyme-Instructed Self-Assembly for Spatiotemporal Profiling of the Activities of Alkaline Phosphatases on Live Cells.
Article in Chem, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 170 citations in OpenAlex.
- Achieving cell-type-specific bioorthogonal chemistry using enzyme-activated caged tetrazines.Nature chemical biology · 2026Article
- Surface Engineered Biomolecular Condensates for Targeted Cell Cytotoxicity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Mechanical forces from intercellular peptide self-assembly drive spheroid formation.Nature communications · 2026Article
- Features and mechanism of localized enzyme-assisted self-assembly of peptides from unilamellar vesicles.Frontiers in chemistry · 2026Article
- Enzyme-Instructed Self-Assembly for Cellular Supramolecular Chemistry.Chemistry, an Asian journal · 2025Review
- Computational Discovery of Aggregation-Mediated Dipeptide Inhibitors Targeting PHLDA1 for Cardiovascular Therapy.ACS omega · 2025Article
- Intrinsically Disordered Peptide Nanofibers from a Structured Motif Within Proteins.Angewandte Chemie (International ed. in English) · 2025Article
- Organelle-Specific Smart Supramolecular Materials for Bioimaging and Theranostics Application.Topics in current chemistry (Cham) · 2024Review
- Phe-Phe-Based Macroscopic Supramolecular Hydrogel Construction Strategies and Biomedical Applications.Chem & bio engineering · 2024Review
- Mapping enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons.Nature methods · 2024Article
- Enzymatic control of intermolecular interactions for generating synthetic nanoarchitectures in cellular environment.Science and technology of advanced materials · 2024Review
- Self-Assembly, Bioactivity, and Nanomaterials Applications of Peptide Conjugates with Bulky Aromatic Terminal Groups.ACS applied bio materials · 2023Review
- Enzyme-instructed morphology transformation of mitochondria-targeting peptide for the selective eradication of osteosarcoma.RSC chemical biology · 2022Article
- Article
- Intracellular Enzyme-Instructed Self-Assembly of Peptides (IEISAP) for Biomedical Applications.Molecules (Basel, Switzerland) · 2022Review
- Perpetuating enzymatically induced spatiotemporal pH and catalytic heterogeneity of a hydrogel by nanoparticles.Chemical science · 2022Article
- Enzyme-mediated intratumoral self-assembly of nanotheranostics for enhanced imaging and tumor therapy.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022Review
- Self-Assembled Peptide Habitats to Model Tumor Metastasis.Gels (Basel, Switzerland) · 2022Article
- Enzymatic Noncovalent Synthesis for Targeting Subcellular Organelles.ChemPlusChem · 2022Review
- Organelle-inspired supramolecular nanomedicine to precisely abolish liver tumor growth and metastasis.Bioactive materials · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Alkaline phosphatase (ALP), an ectoenzyme, plays important roles in biology. But there is no activity probes for imaging ALPs in live cell environment due to the diffusion and cytotoxicity of current probes. Here we report the profiling of the activities of ALPs on live cells by enzyme-instructed self-assembly (EISA) of a D-peptidic derivative that forms fluorescent, non-diffusive nanofibrils. Our study reveals the significantly higher activities of ALP on cancer cells than on stromal cells in their co-culture and shows an inherent and dynamic difference in ALP activities between drug sensitive and resistant cancer cells or between cancer cells with and without hormonal stimulation. Being complementary to genomic profiling of cells, EISA, as a reaction-diffusion controlled process, achieves high spatiotemporal resolution for profiling activities of ALPs of live cells at single cell level. The activity probes of ALP contribute to understanding the reversible phosphorylation/dephosphorylation in the extracellular domains that is an emerging frontier in biomedicine.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.