ArticleStem cell reports2017
Glycolysis-Optimized Conditions Enhance Maintenance of Regenerative Integrity in Mouse Spermatogonial Stem Cells during Long-Term Culture.
Article in Stem cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Metabolic transitions define spermatogonial stem cell maturation.Human reproduction (Oxford, England) · 2022Pooled it
- RUNX proteins and CBFβ create an interconnected network of transcriptional regulation in the male germline†.Biology of reproduction · 2026Article
- SMAD3 and HIF-1α orchestrate metabolic transition to aerobic glycolysis as a critical prerequisite for spontaneous reprogramming of spermatogonial stem cells.Stem cell research & therapy · 2025Article
- Key events in the process of sex determination and differentiation in early chicken embryos.Animal bioscience · 2025Article
- Single-cell multiomic comparison of mouse and rat spermatogenesis reveals gene regulatory networks conserved for over 20 million years.Stem cell reports · 2025Article
- Genome editing in mouse spermatogonial stem cell lines targeting the Tex15 gene using CRISPR/Cas9.Frontiers in veterinary science · 2025Article
- Accelerated mitochondrial dynamics promote spermatogonial differentiation.Stem cell reports · 2024Article
- Integrating Microarray Data and Single-Cell RNA-Seq Reveals Key Gene Involved in Spermatogonia Stem Cell Aging.International journal of molecular sciences · 2024Article
- Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation†.Biology of reproduction · 2024Review
- Autophagy accompanying the developmental process of male germline stem cells.Cell and tissue research · 2024Review
- LncRNA ACVR2B-as1 interacts with ALDOA to regulate the self-renewal and apoptosis of human spermatogonial stem cells by controlling glycolysis activity.Cellular and molecular life sciences : CMLS · 2024Article
- Screening of Reference Genes for Quantitative Real-Time PCR Analysis in Tissues and during Testis Development, and Application to Analyze the Expression ofAnimals : an open access journal from MDPI · 2024Article
- Multiple ageing effects on testicular/epididymal germ cells lead to decreased male fertility in mice.Communications biology · 2024Article
- The hypoxia-inducible factor EPAS1 is required for spermatogonial stem cell function in regenerative conditions.iScience · 2023Article
- Characterization of long-termZoological research · 2023Article
- Glutamine protects mouse spermatogonial stem cells against NOX1-derived ROS for sustaining self-renewal division in vitro.Development (Cambridge, England) · 2023Article
- Transcription factor E4F1 dictates spermatogonial stem cell fate decisions by regulating mitochondrial functions and cell cycle progression.Cell & bioscience · 2023Article
- Ubiquitin protein E3 ligase ASB9 suppresses proliferation and promotes apoptosis in human spermatogonial stem cell line by inducing HIF1AN degradation.Biological research · 2023Article
- Impact of the hypoxic microenvironment on spermatogonial stem cells in culture.Frontiers in cell and developmental biology · 2023Article
- Oxygen availability influences the incidence of testicular teratoma inFrontiers in genetics · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The application of spermatogonial stem cell (SSC) transplantation for regenerating male fertility requires amplification of SSC number in vitro during which the integrity to re-establish spermatogenesis must be preserved. Conventional conditions supporting proliferation of SSCs from mouse pups have been the basis for developing methodology with adult human cells but are unrefined. We found that the integrity to regenerate spermatogenesis after transplantation declines with advancing time in primary cultures of pup SSCs and that the efficacy of deriving cultures from adult SSCs is limited with conventional conditions. To address these deficiencies, we optimized the culture environment to favor glycolysis as the primary bioenergetics process. In these conditions, regenerative integrity of pup and adult SSCs was significantly improved and the efficiency of establishing primary cultures was 100%. Collectively, these findings suggest that SSCs are primed for conditions favoring glycolytic activity, and matching culture environments to their bioenergetics is critical for maintaining functional integrity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.