Evidence map›Paper›PMID 28392095›Full record

ArticleBiochemical pharmacology2017

Real-time analysis of the binding of fluorescent VEGF

Laura E Kilpatrick, Rachel Friedman-Ohana, Diana C Alcobia, Kristin Riching, Chloe J Peach, Amanda J Wheal, Stephen J Briddon, Matthew B Robers, Kris Zimmerman, Thomas Machleidt and 3 more

Open access · hybridAbstract read
In one paragraph

Article in Biochemical pharmacology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 51 citations in OpenAlex.

  1. Review
  2. Article
  3. On the structural identifiability of nonlinear models of ligand binding dynamics.Journal of pharmacokinetics and pharmacodynamics · 2026
    Article
  4. A method for rapid and reliable quantification of VEGF-cell binding activity.Biochemical and biophysical research communications · 2024
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Recent biomedical advances enabled by HaloTag technology.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2022
    Article
  11. Article
  12. Interactions between Ligand-Bound EGFR and VEGFR2.Journal of molecular biology · 2021
    Article
  13. Review
  14. Review
  15. Article
  16. Review
  17. Article
  18. Article
  19. Complex Formation between VEGFR2 and the βCell chemical biology · 2019
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Laura E KilpatrickCell Signalling and Pharmacology Research Group, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
Rachel Friedman-OhanaPromega Corporation, Madison, WI, USA.
Diana C AlcobiaCell Signalling and Pharmacology Research Group, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
Kristin RichingPromega Corporation, Madison, WI, USA.
Chloe J PeachCell Signalling and Pharmacology Research Group, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
Amanda J WhealCell Signalling and Pharmacology Research Group, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
Stephen J BriddonCell Signalling and Pharmacology Research Group, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
Matthew B RobersPromega Corporation, Madison, WI, USA.
Kris ZimmermanPromega Corporation, Madison, WI, USA.
Thomas MachleidtPromega Corporation, Madison, WI, USA.
Keith V WoodPromega Corporation, Madison, WI, USA.
Jeanette WoolardCell Signalling and Pharmacology Research Group, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom. Electronic address: jeanette.woolard@nottingham.ac.uk.
Stephen J HillCell Signalling and Pharmacology Research Group, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom. Electronic address: stephen.hill@nottingham.ac.uk.
University of Nottingham · GBPromega (United States) · US

Funding

Biotechnology and Biological Sciences Research Council BB/L013827/1Biotechnology and Biological Sciences Research Council BB/L019418/1
6 · The paper itself

Abstract

Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis. Here we have used a novel stoichiometric protein-labeling method to generate a fluorescent variant of VEGF (VEGF

Indexed as

Computer SystemsAmino Acid SequenceDose-Response Relationship, DrugEndocytosisFluorescent DyesHEK293 CellsHumansHuman Umbilical Vein Endothelial CellsProtein BindingProtein Kinase InhibitorsRecombinant ProteinsVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2Fluorescent DyesKDR protein, humanProtein Kinase InhibitorsRecombinant ProteinsVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2VEGFA protein, humanBRETCediranib (Pubchem CIC 9933475)Furimazine (Pubchem CIC 219083)Ligand bindingPazopanib (Pubchem CIC 10113978)Receptor tyrosine kinase inhibitorsSorafenib (Pubchem CIC 216239)Vandetanib (Pubchem CIC 3081361)VEGFVEGFR2

Identifiers

PMID28392095
PMCPMC5457915
OpenAlexW2604285083

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.