ArticleFood and environmental virology2017
Evaluation of Two Triplex One-Step qRT-PCR Assays for the Quantification of Human Enteric Viruses in Environmental Samples.
Article in Food and environmental virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- One-year monitoring of potentially pathogenic microorganisms in the waters and sediments of the Lesina and Varano lagoons (South-East Italy).Italian journal of food safety · 2024Article
- Near-source passive sampling for monitoring viral outbreaks within a university residential setting.Epidemiology and infection · 2024Article
- Use of wastewater from passenger ships to assess the movement of COVID-19 and other pathogenic viruses across maritime international boundaries.Frontiers in public health · 2024Article
- Enhanced detection of viruses for improved water safety.Scientific reports · 2023Article
- Evaluating the transmission risk of SARS-CoV-2 from sewage pollution.The Science of the total environment · 2023Article
- Comparative Assessment of Filtration- and Precipitation-Based Methods for the Concentration of SARS-CoV-2 and Other Viruses from Wastewater.Microbiology spectrum · 2022Article
- SARS-CoV-2 in environmental perspective: Occurrence, persistence, surveillance, inactivation and challenges.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- Viral indicators for tracking domestic wastewater contamination in the aquatic environment.Water research · 2020Review
- Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.Frontiers in microbiology · 2020Article
- Norovirus Monitoring in Oysters Using Two Different Extraction Methods.Food and environmental virology · 2019Article
- Seasonal and diurnal surveillance of treated and untreated wastewater for human enteric viruses.Environmental science and pollution research international · 2018Article
- Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Human enteric viruses are responsible for waterborne and shellfish-associated disease outbreaks worldwide. Quantitative reverse transcription PCR (qRT-PCR) is often used to assess the health risks associated with shellfish and environmental water, but viral titres in sediments are less commonly investigated. In this study, we developed and validated two multiplex qRT-PCR assays for aquatic sediment and shellfish samples targeting viruses that are a common cause of gastroenteritis (norovirus GI, GII and hepatitis A virus), two emerging viruses (sapovirus and hepatitis E virus), along with mengovirus (MgV), which is often used as a sample process control for the assessment of RNA extraction efficiency. Singleplex and multiplex assays demonstrated comparable PCR efficiencies and gave reliable results over a wide concentration range. The multiplex assays showed remarkable sensitivity with a limit of detection of 1 RNA copy/µL nucleic acid extract for all target viruses and limits of quantification of 3-18 RNA copies/µL for the targeted human pathogenic viruses and 20-40 RNA copies/µL for MgV. The results demonstrated the veracity of multiplex qRT-PCR for the estimation of viral titres in sediment and shellfish, allowing the rapid assessment of viral infection risks associated with environments exposed to wastewater contamination.
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Registered trials
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