Evidence map›Paper›PMID 28391470›Full record

Article3 Biotech2017

Molecular identification and genetic diversity among Photorhabdus and Xenorhabdus isolates.

Reda E A Moghaieb, Abdelhadi A Abdelhadi, Hanan A El-Sadawy, Nesreen A T Allam, Baiome Abdelmaguid Baiome, Mohamed H Soliman

Open access · hybridAbstract read
In one paragraph

Article in 3 Biotech, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Reda E A MoghaiebDepartment of Genetics and Genetic Engineering Research Center (GERC), Faculty of Agriculture Cairo University, Giza, 12613, Egypt.
Abdelhadi A AbdelhadiDepartment of Genetics and Genetic Engineering Research Center (GERC), Faculty of Agriculture Cairo University, Giza, 12613, Egypt.
Hanan A El-SadawyParasitology and Animal Diseases Department, National Research Centre, Elbuhoth St., Dokki, Giza, 12311, Egypt.
Nesreen A T AllamParasitology and Animal Diseases Department, National Research Centre, Elbuhoth St., Dokki, Giza, 12311, Egypt.
Baiome Abdelmaguid BaiomeParasitology and Animal Diseases Department, National Research Centre, Elbuhoth St., Dokki, Giza, 12311, Egypt.
Mohamed H SolimanDepartment of Genetics and Genetic Engineering Research Center (GERC), Faculty of Agriculture Cairo University, Giza, 12613, Egypt. mohamed.soliman1919@gmail.com.
National Research Centre · EGCairo University · EGUniversity of Modern Sciences · AE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria mellonella) infected with the entomopathogenic nematodes: Heterorhabditis bacteriophora HP88, Heterorhabditis indicus RM1 and Heterorhabditis sp (S1), Steinernema abbasi and Steinernema sp. (S II). Strains were identified as Photorhabdus luminescens HRM1, P. luminescens HS1, P. luminescens HP88, Xenorhabdus indica and X. nematophila ATTC19061 using 16S rDNA sequence analysis. To reveal the genetic diversity among these strains, three molecular markers (RAPD, ISSR and SRAP) were employed. RAPD analysis showed 73.8 and 54.5 polymorphism percentages for the Photorhabdus and Xenorhabdus strains, respectively. ISSR analysis resulted in 70.1 and 75.2 polymorphism percentages among the Photorhabdus and Xenorhabdus strains, respectively. The SRAP analysis indicated that 75.6 and 61.2% genetic polymorphism was detected among Photorhabdus and Xenorhabdus strains, respectively. The cluster analysis grouped the three Photorhabdus strains together in one cluster and the two Xenorhabdus strains together in another cluster indicating the phylogenetic relationships among them. The genotype-specific markers detected from the three molecular markers (RAPD, ISSR and SRAP) were sufficient to distinguish between the different bacterial strains tested and can be used in the future IBM program that could be built on the use of these strains.

Indexed as

16S rDNAGenetic diversityMolecular markersPhotorhabdusXenorhabdus

Identifiers

PMID28391470
PMCPMC5385175
OpenAlexW2604660565

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.