ArticleCellular and molecular life sciences : CMLS2017
Ultrastructural localization of 5-methylcytosine on DNA and RNA.
Article in Cellular and molecular life sciences : CMLS, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Visualizing Epigenetics: A Review of Microscopy Techniques for Investigating DNA Methylation Patterns, Chromatin Structure, and Gene Expression.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2025Review
- FusDRM-m5C: a hybrid model for accurate prediction of 5-methylcytosine modification sites based on feature fusion and attention mechanism.Frontiers in genetics · 2025Article
- MSCAN: multi-scale self- and cross-attention network for RNA methylation site prediction.BMC bioinformatics · 2024Article
- Acute Transcriptomic and Epigenetic Alterations at T12 After Rat T10 Spinal Cord Contusive Injury.Molecular neurobiology · 2023Article
- N6-methyladenine-modified DNA was decreased in Alzheimer's disease patients.World journal of clinical cases · 2022Article
- Article
- Ultrastructural immunocytochemistry shows impairment of RNA pathways in skeletal muscle nuclei of old mice: A link to sarcopenia?European journal of histochemistry : EJH · 2021Article
- Prediction of m5C Modifications in RNA Sequences by Combining Multiple Sequence Features.Molecular therapy. Nucleic acids · 2020Article
- Squaring the Circle: A New Study of Inward and Outward-Rectifying Potassium Currents in U251 GBM Cells.Cellular and molecular neurobiology · 2020Article
- Regulation of pluripotency and reprogramming by RNA binding proteins.Current topics in developmental biology · 2020Review
- Ribosomal DNA and the Nucleolus as Keystones of Nuclear Architecture, Organization, and Function.Trends in genetics : TIG · 2019Review
- Detection and analysis of RNA methylation.F1000Research · 2019Review
- Perichromatin region: a moveable feast.Histochemistry and cell biology · 2018Review
- Nucleolus and chromatin.Histochemistry and cell biology · 2018Review
- RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells.Nature genetics · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is the major epigenetic modification and it is involved in the negative regulation of gene expression. Its alteration can lead to neoplastic transformation. Several biomolecular approaches are nowadays used to study this modification on DNA, but also on RNA molecules, which are known to play a role in different biological processes. RNA methylation is one of the most common RNA modifications and 5-methylcytosine presence has recently been suggested in mRNA. However, an analysis of nucleic acid methylation at electron microscope is still lacking. Therefore, we visualized DNA methylation status and RNA methylation sites in the interphase nucleus of HeLa cells and rat hepatocytes by ultrastructural immunocytochemistry and cytochemical staining. This approach represents an efficient alternative to study nucleic acid methylation. In particular, this ultrastructural method makes the visualization of this epigenetic modification on a single RNA molecule possible, thus overcoming the technical limitations for a (pre-)mRNA methylation analysis.
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Registered trials
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