Evidence map›Paper›PMID 28389839›Full record

ArticleInternational orthopaedics2017

P15 peptide stimulates chondrogenic commitment and endochondral ossification.

Jun Zhang, Peter Eisenhauer, Ozҫan Kaya, Alexander R Vaccaro, Carol Diallo, Andrzej Fertala, Theresa A Freeman

Abstract read
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In one paragraph

Article in International orthopaedics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Bone Grafts: Everything You Need to Know.Journal of periodontal research · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Jun ZhangSidney Kimmel Medical College Department of Orthopaedic Surgery, Thomas Jefferson University, 501 Curtis Bldg., 1015 Walnut St., Philadelphia, PA, 19107, USA.
Peter EisenhauerRothman Institute at Jefferson, 925 Chestnut Street, Philadelphia, PA, 19107, USA.
Ozҫan KayaDepartment of Orthopedics and Traumatology, Istanbul University, Istanbul, 34093, Turkey.
Alexander R VaccaroSidney Kimmel Medical College Department of Orthopaedic Surgery, Thomas Jefferson University, 501 Curtis Bldg., 1015 Walnut St., Philadelphia, PA, 19107, USA.
Carol DialloSidney Kimmel Medical College Department of Orthopaedic Surgery, Thomas Jefferson University, 501 Curtis Bldg., 1015 Walnut St., Philadelphia, PA, 19107, USA.
Andrzej FertalaSidney Kimmel Medical College Department of Orthopaedic Surgery, Thomas Jefferson University, 501 Curtis Bldg., 1015 Walnut St., Philadelphia, PA, 19107, USA.
Theresa A FreemanSidney Kimmel Medical College Department of Orthopaedic Surgery, Thomas Jefferson University, 501 Curtis Bldg., 1015 Walnut St., Philadelphia, PA, 19107, USA. Theresa.freeman@jefferson.edu.
Thomas Jefferson University · USIstanbul University · TRRothman Institute · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe synthetic 15 amino acid biomimetic peptide sequence (P15) derived from a region of the alpha (α)-1 chain of collagen I, has been shown to promote α2 integrin activation and enhance intramembranous ossification. In this study, we ask if the P15 peptide also enhances bone formation through endochondral ossification, and determine if direct binding of α2 integrin with P15 mediates integrin activation.

methodsMesenchymal cells (C3H10T1/2) were cultured in chondrogenic media and the expression of chondrogenic markers and integrin activation was determined by Western blot and fluorescent immunohistochemistry. A biosensor assay was used to determine if binding occurred between P15 and α2 β1 integrin. Finally, an in vivo model of endochondral ossification was used to determine the effect of P15 on bone formation.

resultsIn the presence of P15, chondrogenesis and activation of α5 integrin were enhanced, as observed by both Western blot analysis and immunoflourescent staining. A biosensor assay investigating the specificity of the interaction between P15 with α2β1 integrin determined direct binding does not occur. When P15 was added to Matrigel implanted in a murine endochondral ossification model, in the presence of bone morphogenic protein-2 (BMP-2), a significant increase in chondrocyte differentiation and mineralization was observed.

conclusionP15 does not directly activate integrins by binding, but does upregulate integrin signaling to enhance differentiation of both osteoblasts and chondrocytes to increase both intramembranous and endochondral bone formation.

Indexed as

AnimalsBiosensing TechniquesBlotting, WesternBone Morphogenetic Protein 2Cell Culture TechniquesCell DifferentiationChondrocytesChondrogenesisCollagenDrug CombinationsFluorescent Antibody TechniqueIntegrinsLamininMesenchymal Stem CellsMiceOsteoblastsBMP2 protein, humanBone Morphogenetic Protein 2cell-binding peptide P-15CollagenDrug CombinationsIntegrinsLamininmatrigelPeptide FragmentsProteoglycansBone densityChondrogenesisCollagenEndochondral ossificationIntegrinMicroCT analysisP15 peptide

Identifiers

PMID28389839
OpenAlexW2605336765

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.