ArticleScientific reports2017
Nanodiamonds as multi-purpose labels for microscopy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 94 citations in OpenAlex.
- Follicle-stimulating hormone stimulates free radical generation without inducing substantial oxidative stress in human granulosa cells.Human reproduction open · 2025Article
- Free radical detection in precision-cut mouse liver slices with diamond-based quantum sensing.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Investigations on anticancer activity of EuHeliyon · 2024Article
- Timing and Mechanisms of Nanodiamond Uptake in Colon Cancer Cells.Nanotechnology, science and applications · 2024Article
- Nanodiamond Effects on Cancer Cell Radiosensitivity: The Interplay between Their Chemical/Physical Characteristics and the Irradiation Energy.International journal of molecular sciences · 2023Article
- Quantum Sensing of Free Radicals in Primary Human Granulosa Cells with Nanoscale Resolution.ACS central science · 2023Article
- Fluorescent Nanodiamonds for Tracking Single Polymer Particles in Cells and Tissues.Analytical chemistry · 2023Article
- Intracellular Quantum Sensing of Free-Radical Generation Induced by Acetaminophen (APAP) in the Cytosol, in Mitochondria and the Nucleus of Macrophages.ACS sensors · 2022Article
- Intracellular Relaxometry, Challenges, and Future Directions.ACS central science · 2022Review
- Functionalized Fluorescent Nanodiamonds for Simultaneous Drug Delivery and Quantum Sensing in HeLa Cells.ACS applied materials & interfaces · 2022Article
- Insight into a Fenton-like Reaction Using Nanodiamond Based Relaxometry.Nanomaterials (Basel, Switzerland) · 2022Article
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- Following Polymer Degradation with Nanodiamond Magnetometry.ACS sensors · 2022Article
- One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM.Frontiers in cell and developmental biology · 2022Review
- Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes.Frontiers in cell and developmental biology · 2022Article
- Fluorescent Nanodiamonds for Detecting Free-Radical Generation in Real Time during Shear Stress in Human Umbilical Vein Endothelial Cells.ACS sensors · 2021Article
- Fluorescent nanodiamonds for characterization of nonlinear microscopy systems.Photonics research · 2021Article
- Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures.Nature protocols · 2021Article
- Harnessing nanotechnology to expand the toolbox of chemical biology.Nature chemical biology · 2021Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This interest stems from (i) strong resistance to photobleaching allowing prolonged fluorescence observation times; (ii) the possibility to excite fluorescence using a focused electron beam (cathodoluminescence; CL) for high-resolution localization; and (iii) the potential use for nanoscale sensing. For all these schemes, the development of versatile molecular labeling using relatively small diamonds is essential. Here, we show the direct targeting of a biological molecule with nanodiamonds as small as 70 nm using a streptavidin conjugation and standard antibody labelling approach. We also show internalization of 40 nm sized nanodiamonds. The fluorescence from the nanodiamonds survives osmium-fixation and plastic embedding making them suited for correlative light and electron microscopy. We show that CL can be observed from epon-embedded nanodiamonds, while surface-exposed nanoparticles also stand out in secondary electron (SE) signal due to the exceptionally high diamond SE yield. Finally, we demonstrate the magnetic read-out using fluorescence from diamonds prior to embedding. Thus, our results firmly establish nanodiamonds containing nitrogen-vacancy centers as unique, versatile probes for combining and correlating different types of microscopy, from fluorescence imaging and magnetometry to ultrastructural investigation using electron microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.