ArticleJournal of cell science2017
Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis.
Article in Journal of cell science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 50 citations in OpenAlex.
- Low kinetochore-microtubule occupancy leaves a mitotic memory by delaying checkpoint silencing.Nature communications · 2026Article
- Nondisruptive inducible labeling of ER-membrane contact sites using the Lamin B receptor.PLoS biology · 2025Article
- Structural characterization and inhibition of the interaction between ch-TOG and TACC3.The Journal of cell biology · 2025Article
- The Kinesin-14 tail: Dual microtubule binding domains drive spindle morphogenesis through tight microtubule cross-linking and robust sliding.Molecular biology of the cell · 2025Article
- An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment.Journal of cell science · 2024Article
- Long-range repulsion between chromosomes in mammalian oocyte spindles.Science advances · 2024Article
- 3D Ultrastructural Visualization of Mitosis Fidelity in Human Cells Using Serial Block Face Scanning Electron Microscopy (SBF-SEM).Bio-protocol · 2023Article
- A tubule-sheet continuum model for the mechanism of nuclear envelope assembly.Developmental cell · 2023Article
- Article
- Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes.The Journal of cell biology · 2022Article
- Non-centrosomal microtubules at kinetochores promote rapid chromosome biorientation during mitosis in human cells.Current biology : CB · 2022Article
- Anaphase B: Long-standing models meet new concepts.Seminars in cell & developmental biology · 2021Review
- Role of spatial patterns and kinetochore architecture in spindle morphogenesis.Seminars in cell & developmental biology · 2021Review
- A belt for the cell: cellulosic wall thickenings and their role in morphogenesis of the 3D puzzle cells in walnut shells.Journal of experimental botany · 2021Article
- Moonlighting of mitotic regulators in cilium disassembly.Cellular and molecular life sciences : CMLS · 2021Review
- CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6.Communications biology · 2021Article
- Mitotic spindle: lessons from theoretical modeling.Molecular biology of the cell · 2021Article
- Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.Journal of cell science · 2021Article
- 3D FIB-SEM reconstruction of microtubule-organelle interaction in whole primary mouse β cells.The Journal of cell biology · 2021Article
- The coordination of nuclear envelope assembly and chromosome segregation in metazoans.Nucleus (Austin, Tex.) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Serial block face scanning electron microscopy (SBF-SEM) is a powerful method to analyze cells in 3D. Here, working at the resolution limit of the method, we describe a correlative light-SBF-SEM workflow to resolve microtubules of the mitotic spindle in human cells. We present four examples of uses for this workflow that are not practical by light microscopy and/or transmission electron microscopy. First, distinguishing closely associated microtubules within K-fibers; second, resolving bridging fibers in the mitotic spindle; third, visualizing membranes in mitotic cells, relative to the spindle apparatus; and fourth, volumetric analysis of kinetochores. Our workflow also includes new computational tools for exploring the spatial arrangement of microtubules within the mitotic spindle. We use these tools to show that microtubule order in mitotic spindles is sensitive to the level of TACC3 on the spindle.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.