ArticleInfectious diseases of poverty2017
A novel colloidal gold immunochromatography assay strip for the diagnosis of schistosomiasis japonica in domestic animals.
Article in Infectious diseases of poverty, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Diagnosis of Schistosoma infection in non-human animal hosts: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2022Pooled it
- Development of lateral flow assays for the rapid diagnosis of glanders in equids.Microbiology spectrum · 2026Article
- Development of a colloidal gold immunochromatographic strip for the rapid detection of fowl adenovirus serotype 4 using prepared penton protein-specific monoclonal antibodies.Frontiers in veterinary science · 2026Article
- Development of an Indirect ELISA and a Microsphere Detection Test Strip for the Detection of Spirometra mansoni.Transboundary and emerging diseases · 2026Article
- Rapid identification of A29L antibodies based on mRNA immunization and high-throughput single B cell sequencing to detect Monkeypox virus.Emerging microbes & infections · 2024Article
- Highly sensitive time-resolved fluorescent microspheres lateral flow immunoassay for the quantitative detection of triadimefon and its metabolite residues in fruits and vegetables.Mikrochimica acta · 2024Article
- Article
- Comparative assessment of the SjSAP4-incorporated gold immunochromatographic assay for the diagnosis of human schistosomiasis japonica.Frontiers in public health · 2023Article
- Development and assessment of a novel gold immunochromatographic assay for the diagnosis of schistosomiasis japonica.Frontiers in immunology · 2023Article
- Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies againstFrontiers in veterinary science · 2022Article
- Potential application of nanotechnology in the treatment, diagnosis, and prevention of schistosomiasis.Frontiers in bioengineering and biotechnology · 2022Review
- Environmental Drivers and Potential Distribution ofMicroorganisms · 2021Article
- The next-generation coronavirus diagnostic techniques with particular emphasis on the SARS-CoV-2.Journal of medical virology · 2021Review
- Establishment of a fluorescence staining method for Schistosoma japonicum miracidia.Scientific reports · 2020Article
- Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks.Poultry science · 2020Article
- Elimination of schistosomiasis: the tools required.Infectious diseases of poverty · 2017Article
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Authors and funding
16 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSchistosomiasis remains a major public health concern in China and an epidemiological survey has revealed that schistosome-infected bovines and goats are the main transmission sources for the disease. Therefore, development of a sensitive technique for the diagnosis of schistosomiasis in domestic animals is necessary.
methodA novel colloidal gold immunochromatography assay (GICA) strip was developed for detecting Schistosoma japonicum in domestic animals. The colloidal gold was conjugated with recombinant streptococcal protein G (rSPG). As the test and control lines, the schistosome soluble egg antigen and rSPG, respectively, were blotted on nitrocellulose membrane.
resultsThe lowest detectable serum dilution was 1∶640 for schistosome-infected buffaloes. The cross-reaction rate of GICA was 14.29% with Paramphistomum sp. in buffaloes, 16.67% with Haemonchus sp. in goats, and 33.33% with Orientobilharzia sp. in goats. These results were slightly lower and similar to those obtained through ELISA. Moreover, the strips for detecting S. japonicum in mice, rabbits, buffaloes, and goats showed high sensitivity (100.00%, 100.00%, 100.00%, and 100.00%, respectively) and specificity (100.00%, 100.00%, 94.23%, and 88.64%, respectively). And the sensitivity or specificity of the GICA strips did not present any significant differences after storage for 12 months at room temperature. When compared with ELISA, the GICA strips exhibited similar sensitivity and specificity in the diagnosis of schistosomiasis in mice, rabbits, buffaloes, and goats. Besides, only 5 μl of serum are required for the test and the detection can be completed within 5 min.
conclusionThis study is the first to develop a GICA strip using gold-rSPG conjugate for the diagnosing of schistosomiasis in domestic animals, and preliminary results showed that the developed strip may be suitable for large-scale screening of schistosomiasis in endemic areas.
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