Evidence map›Paper›PMID 28388934›Full record

ArticleVirology journal2017

Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China.

Jialin Zhang, Xiaoqian Tang, Xiuzhen Sheng, Jing Xing, Wenbin Zhan

Open access · goldAbstract read
In one paragraph

Article in Virology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 49 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jialin ZhangLaboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, China.
Xiaoqian TangLaboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, China. tangxq@ouc.edu.cn.
Xiuzhen ShengLaboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, China.
Jing XingLaboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, China.
Wenbin ZhanLaboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, China.
Ocean University of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe.

methodsIn this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing.

resultsThe gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD

conclusionIn conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV.

Indexed as

AnimalsAquacultureChinaFish DiseasesFlounderMicroscopy, ElectronNovirhabdovirusPhylogenyReverse Transcriptase Polymerase Chain ReactionRhabdoviridae InfectionsRNA, ViralSequence Analysis, DNAVirionRNA, ViralHirame rhabdovirusIdentificationParalichthys olivaceusPathogenicity

Identifiers

PMID28388934
PMCPMC5384145
OpenAlexW2604761260

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.