ArticlePloS one2017
Transcriptomic and physiological analyses of Medicago sativa L. roots in response to lead stress.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 39 citations in OpenAlex.
- Crosstalk between glutathione and melatonin in chromium detoxification in sweet potato revealed by high-throughput sequencing and physio-biochemical profiling.Frontiers in plant science · 2026Article
- Review
- Insights into the molecular mechanisms of browning tolerance in luffa: a transcriptome and metabolome analysis.Frontiers in plant science · 2025Article
- Underlying mechanism of Dendrobium huoshanense resistance to lead stress using the quantitative proteomics method.BMC plant biology · 2024Article
- Pb uptake, accumulation, and translocation in plants: Plant physiological, biochemical, and molecular response: A review.Heliyon · 2024Review
- Physiological, proteomic, and metabolomic analysis provide insights into Bacillus sp.-mediated salt tolerance in wheat.Plant cell reports · 2022Article
- Changes induced by lead in root system architecture of Arabidopsis seedlings are mediated by PDR2-LPR1/2 phosphate dependent way.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2021Article
- Pb Stress and Ectomycorrhizas: Strong Protective Proteomic Responses in Poplar Roots Inoculated withInternational journal of molecular sciences · 2021Article
- Metal and Metalloid Toxicity in Plants: An Overview on Molecular Aspects.Plants (Basel, Switzerland) · 2021Review
- Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa.Frontiers in plant science · 2020Review
- The Overexpression of a Transcription Factor GeneFrontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lead (Pb) is one of the nonessential and toxic metals that threaten the environment and human health. Medicago sativa L. is a legume with high salt tolerance and high biomass production. It is not only a globally important forage crop but is also an ideal plant for phytoremediation. However, the biological and molecular mechanisms that respond to heavy metals are still not well defined in M. sativa. In this study, de novo and strand-specific RNA-sequencing was performed to identify genes involved in the Pb stress response in M. sativa roots. A total of 415,350 unigenes were obtained from the assembled cDNA libraries, among which 5,416 were identified as significantly differentially expressed genes (DEGs) (false discovery rate < 0.005) between cDNA libraries from control and Pb-treated plants. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the DEGs showed they mainly clustered with terms associated with binding, transport, membranes, and the pathways related to signal and energy metabolism. Moreover, a number of candidate genes included antioxidant enzymes, metal transporters, and transcription factors involved in heavy metal response were upregulated under Pb stress. Quantitative real-time PCR(qRT-PCR) validation of the expression patterns of 10 randomly selected candidate DEGs were consistent with the transcriptome analysis results. Thus, this study offers new information towards the investigation of biological changes and molecular mechanisms related to Pb stress response in plants.
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