ArticleNucleic acids research2017
Achieving large dynamic range control of gene expression with a compact RNA transcription-translation regulator.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Compact RNA sensors for increasingly complex functions of multiple inputs.Nature chemistry · 2025Article
- Population-level amplification of gene regulation by programmable gene transfer.Nature chemical biology · 2025Article
- Generative and predictive neural networks for the design of functional RNA molecules.Nature communications · 2025Article
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- Inducible plasmid copy number control for synthetic biology in commonly used E. coli strains.Nature communications · 2022Article
- Engineering Toehold-Mediated Switches for Native RNA Detection and Regulation in Bacteria.Journal of molecular biology · 2022Review
- Engineering synthetic RNA devices for cell control.Nature reviews. Genetics · 2022Review
- Attenuator LRR - a regulatory tool for modulating gene expression in Gram-positive bacteria.Microbial biotechnology · 2021Article
- A small-molecule chemical interface for molecular programs.Nucleic acids research · 2021Article
- Dynamic Control of Gene Expression with Riboregulated Switchable Feedback Promoters.ACS synthetic biology · 2021Article
- Harnessing the central dogma for stringent multi-level control of gene expression.Nature communications · 2021Article
- A suppressor tRNA-mediated feedforward loop eliminates leaky gene expression in bacteria.Nucleic acids research · 2021Article
- Programmable cross-ribosome-binding sites to fine-tune the dynamic range of transcription factor-based biosensor.Nucleic acids research · 2020Article
- Regulatory systems for gene expression control in cyanobacteria.Applied microbiology and biotechnology · 2020Review
- Extremely Low Leakage Expression Systems Using Dual Transcriptional-Translational Control for Toxic Protein Production.International journal of molecular sciences · 2020Review
- Advances in engineered trans-acting regulatory RNAs and their application in bacterial genome engineering.Journal of industrial microbiology & biotechnology · 2019Review
- Engineering orthogonal synthetic timer circuits based on extracytoplasmic function σ factors.Nucleic acids research · 2018Article
- Engineering Diagnostic and Therapeutic Gut Bacteria.Microbiology spectrum · 2017Review
- Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation.Biotechnology advancesReview
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA transcriptional regulators are emerging as versatile components for genetic network construction. However, these regulators suffer from incomplete repression in their OFF state, making their dynamic range less than that of their protein counterparts. This incomplete repression causes expression leak, which impedes the construction of larger synthetic regulatory networks as leak propagation can interfere with desired network function. To address this, we demonstrate how naturally derived antisense RNA-mediated transcriptional regulators can be configured to regulate both transcription and translation in a single compact RNA mechanism that functions in Escherichia coli. Using in vivo gene expression assays, we show that a combination of transcriptional termination and ribosome binding site sequestration increases repression from 85% to 98%, or activation from 10-fold to over 900-fold, in response to cognate antisense RNAs. We also show that orthogonal repressive versions of this mechanism can be created through engineering minimal antisense RNAs. Finally, to demonstrate the utility of this mechanism, we use it to reduce network leak in an RNA-only cascade. We anticipate these regulators will find broad use as synthetic biology moves beyond parts engineering to the design and construction of more sophisticated regulatory networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.