Evidence map›Paper›PMID 28387379›Full record

ArticleScientific reports2017

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices.

Koji Matsuura, Han-Wei Huang, Ming-Cheng Chen, Yu Chen, Chao-Min Cheng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.7field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
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  6. Review
  7. Article
  8. Machine Learning Approach for Muscovy Duck (Animals : an open access journal from MDPI · 2023
    Article
  9. Article
  10. Sperm Gone Smart: A Portable Device (iSpermAnimals : an open access journal from MDPI · 2022
    Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. The Roles of NO and HInternational journal of molecular sciences · 2020
    Review
  16. Article
  17. Review
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Koji MatsuuraDepartment of Biomedical Engineering, Faculty of Engineering, Okayama University of Science, 1-1 Ridai-Cho Kita-Ku, Okayama, 700-0005, Japan.
Han-Wei HuangInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Ming-Cheng ChenInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Yu ChenDepartment of Urology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan 33302, Taiwan.
Chao-Min ChengInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
National Tsing Hua University · TWChang Gung Memorial Hospital · TWOkayama University of Science · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mammalian sperm motility has traditionally been analyzed to determine fertility using computer-assisted semen analysis (CASA) systems. To develop low-cost and robust male fertility diagnostics, we created a paper-based MTT assay and used it to estimate motile sperm concentration. When porcine sperm motility was inhibited using sperm enzyme inhibitors for sperm enzymes related to mitochondrial activity and glycolysis, we simultaneously recorded sperm motility and enzymatic reactivity using a portable motility analysis system (iSperm) and a paper-based MTT assay, respectively. When using our paper-based MTT-assay, we calculated the area mean value signal intensity (AMV) to evaluate enzymatic reactivity. Both sperm motility and AMV decreased following treatment with iodoacetamide (IODO) and 3-bromopyruvic acid (3BP), both of which are inhibitors of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We found a correlation between recorded motility using iSperm and AMV from our paper-based assay (P < 0.05), suggesting that a sperm-related enzymatic reaction is involved in sperm motility. Under this protocol, MTT reduction was coupled with catalysis of GAPDH and was promoted by electron transfer from NADH. Based on this inhibitor study, sperm motility can be estimated using our paper-based MTT-assay.

Indexed as

Semen AnalysisSperm MotilityAdenosine TriphosphateAnimalsBiomarkersDose-Response Relationship, DrugGlyceraldehyde 3-PhosphateGlyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)MaleMitochondriaSpermatozoaSwineAdenosine TriphosphateBiomarkersGlyceraldehyde 3-PhosphateGlyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)

Identifiers

PMID28387379
PMCPMC5384208
OpenAlexW2605335820

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.