ArticleScientific reports2017
Multi-color electron microscopy by element-guided identification of cells, organelles and molecules.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Islet ultrastructure: past achievements and future directions.Diabetologia · 2026Review
- From lipofuscin accumulation to cellular dysfunction: a focus on liver pathophysiology.Histochemistry and cell biology · 2026Review
- Mitigation of artifacts in imaging biosamples with optical scanning transmission electron microscopy.Applied microscopy · 2026Article
- Automated analysis of ultrastructure through large-scale hyperspectral electron microscopy.Npj imaging · 2024Article
- Liquid Phase Electron Microscopy of Bacterial Ultrastructure.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Cathodoluminescent and Characteristic X-Ray-Emissive Rare-Earth-Doped Core/Shell Protein Labels for Spectromicroscopic Analysis of Cell Surface Receptors.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Morphomics via next-generation electron microscopy.Journal of molecular cell biology · 2024Review
- Spatial Multiplexing and Omics.Nature reviews. Methods primers · 2024Article
- Cloneable inorganic nanoparticles.Chemical communications (Cambridge, England) · 2023Review
- Biocompatibility and degradation comparisons of four biodegradable copolymeric osteosynthesis systems used in maxillofacial surgery: A goat model with four years follow-up.Bioactive materials · 2022Article
- Development and Application of a Semi quantitative Scoring Method for Ultrastructural Assessment of Acute Stress in Pancreatic Islets.Transplantation direct · 2022Article
- The Human Islet: Mini-Organ With Mega-Impact.Endocrine reviews · 2021Review
- Elemental mapping of labelled biological specimens at intermediate energy loss in an energy-filtered TEM acquired using a direct detection device.Journal of microscopy · 2021Article
- State-of-the-art microscopy to understand islets of Langerhans: what to expect next?Immunology and cell biology · 2021Review
- Pancreatic beta cell autophagy is impaired in type 1 diabetes.Diabetologia · 2021Article
- Article
- Cryo-fluorescence microscopy of high-pressure frozen C. elegans enables correlative FIB-SEM imaging of targeted embryonic stages in the intact worm.Methods in cell biology · 2021Article
- Article
- Large-scale electron microscopy database for human type 1 diabetes.Nature communications · 2020Article
- Morphological peculiarities of the DNA-protein complexes in starved Escherichia coli cells.PloS one · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular complexity is unraveled at nanometer resolution using electron microscopy (EM), but interpretation of macromolecular functionality is hampered by the difficulty in interpreting grey-scale images and the unidentified molecular content. We perform large-scale EM on mammalian tissue complemented with energy-dispersive X-ray analysis (EDX) to allow EM-data analysis based on elemental composition. Endogenous elements, labels (gold and cadmium-based nanoparticles) as well as stains are analyzed at ultrastructural resolution. This provides a wide palette of colors to paint the traditional grey-scale EM images for composition-based interpretation. Our proof-of-principle application of EM-EDX reveals that endocrine and exocrine vesicles exist in single cells in Islets of Langerhans. This highlights how elemental mapping reveals unbiased biomedical relevant information. Broad application of EM-EDX will further allow experimental analysis on large-scale tissue using endogenous elements, multiple stains, and multiple markers and thus brings nanometer-scale 'color-EM' as a promising tool to unravel molecular (de)regulation in biomedicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.