ArticleScientific reports2017
Targeting microbial biofilms using Ficin, a nonspecific plant protease.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 127 citations in OpenAlex.
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- Beta Carboline Alkaloid Harmine as Biofilm Inhibitor: In vitro, in Silico and in Vivo Studies Suppressing Growth and Virulence-Related Factors Against Resistant Staphylococcus Aureus.Applied biochemistry and biotechnology · 2026Article
- Targeting Bacterial Biofilms on Medical Implants: Current and Emerging Approaches.Antibiotics (Basel, Switzerland) · 2025Review
- Production and Potential Application of an Alkaline Serine Peptidase from Myceliophtora heterothallica for Biofilm Removal.Current microbiology · 2025Article
- New BacteriophageViruses · 2025Article
- Various Options for Covalent Immobilization of Cysteine Proteases-Ficin, Papain, Bromelain.International journal of molecular sciences · 2025Article
- Dishwashing liquids with nuclease and protease: An improved biocompatible solution for the removal of adherent bacteria from fruits and vegetables.AIMS microbiology · 2025Article
- Article
- Plant-Derived Proteins and Peptides as Potential Immunomodulators.Molecules (Basel, Switzerland) · 2023Article
- The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens.International journal of molecular sciences · 2023Article
- Ficin-Cyclodextrin-Based Docking Nanoarchitectonics of Self-Propelled Nanomotors for Bacterial Biofilm Eradication.Chemistry of materials : a publication of the American Chemical Society · 2023Article
- The degradation of chicken feathers by3 Biotech · 2023Article
- Repression ofMicroorganisms · 2023Article
- Alterations in Antibiotic Susceptibility ofInternational journal of molecular sciences · 2023Article
- The Novel Chiral 2(5Molecules (Basel, Switzerland) · 2023Article
- ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells.Medicina (Kaunas, Lithuania) · 2023Article
- Fig latex inhibits the growth of pathogenic bacteria invading human diabetic wounds and accelerates wound closure in diabetic mice.Scientific reports · 2022Article
- Increasing the Efficacy of Treatment ofAntibiotics (Basel, Switzerland) · 2022Article
- Novel Biocatalysts Based on Bromelain Immobilized on Functionalized Chitosans and Research on Their Structural Features.Polymers · 2022Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilms, the communities of surface-attached bacteria embedded into extracellular matrix, are ubiquitous microbial consortia securing the effective resistance of constituent cells to environmental impacts and host immune responses. Biofilm-embedded bacteria are generally inaccessible for antimicrobials, therefore the disruption of biofilm matrix is the potent approach to eradicate microbial biofilms. We demonstrate here the destruction of Staphylococcus aureus and Staphylococcus epidermidis biofilms with Ficin, a nonspecific plant protease. The biofilm thickness decreased two-fold after 24 hours treatment with Ficin at 10 μg/ml and six-fold at 1000 μg/ml concentration. We confirmed the successful destruction of biofilm structures and the significant decrease of non-specific bacterial adhesion to the surfaces after Ficin treatment using confocal laser scanning and atomic force microscopy. Importantly, Ficin treatment enhanced the effects of antibiotics on biofilms-embedded cells via disruption of biofilm matrices. Pre-treatment with Ficin (1000 μg/ml) considerably reduced the concentrations of ciprofloxacin and bezalkonium chloride required to suppress the viable Staphylococci by 3 orders of magnitude. We also demonstrated that Ficin is not cytotoxic towards human breast adenocarcinoma cells (MCF7) and dog adipose derived stem cells. Overall, Ficin is a potent tool for staphylococcal biofilm treatment and fabrication of novel antimicrobial therapeutics for medical and veterinary applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.