Evidence map›Paper›PMID 28386666›Full record

ArticleArchives of microbiology2017

Electrophoretic profiles of lipopolysaccharides from Rhizobium strains nodulating Pisum sativum do not reflect phylogenetic relationships between these strains.

Jolanta Kutkowska, Monika Marek-Kozaczuk, Jerzy Wielbo, Marek Wójcik, Teresa Urbanik-Sypniewska

Open access · hybridAbstract read
In one paragraph

Article in Archives of microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Jolanta KutkowskaDepartment of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland. jolanta.kutkowska@poczta.umcs.lublin.pl.ORCID http://orcid.org/0000-0002-3307-591X
Monika Marek-KozaczukDepartment of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
Jerzy WielboDepartment of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
Marek WójcikDepartment of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
Teresa Urbanik-SypniewskaDepartment of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
Maria Curie-Skłodowska University · PLMedical University of Lublin · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rhizobia that nodulate peas comprise a heterogeneous group of bacteria. The aim of this study was to investigate the relationship between phylogeny and electrophoretic and hydroxy fatty acid lipopolysaccharide (LPS) profiles of pea microsymbionts. Based on amplified fragment length polymorphism (AFLP) fingerprinting data, the pea microsymbionts were grouped into two clusters distinguished at 58% similarity level. Based on the concatenated 16S rRNA, recA, and atpD housekeeping gene data, the microsymbionts appeared to be most closely related to Rhizobium leguminosarum biovars viciae and trifolii. Applying cluster analysis to their LPS electrophoretic profiles, the strains were assigned to two major groups with different banding patterns. All hydroxy fatty acids common to R. leguminosarum and R. etli were detected in each examined strain. Differences in the proportions of 3- to ω-1 hydroxy fatty acids allowed us to distinguish two groups of strains. This classification did not overlap with one based on LPS electrophoretic profiles. No clear correlation was apparent between the genetic traits and LPS profiles of the pea nodule isolates.

Indexed as

Rhizobium leguminosarumAmplified Fragment Length Polymorphism AnalysisBase SequenceDNA, BacterialFatty AcidsLipopolysaccharidesMembrane ProteinsPhylogenyPisum sativumRec A RecombinasesRNA, Ribosomal, 16SRoot Nodules, PlantSequence Analysis, DNASymbiosisDNA, BacterialFatty AcidsLipopolysaccharidesMembrane ProteinsRec A RecombinasesRNA, Ribosomal, 16S16S rRNAAFLP fingerprintingFatty acidsLipopolysaccharidesPisum sativumRhizobium leguminosarum

Identifiers

PMID28386666
PMCPMC5548859
OpenAlexW2604164812

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.