ReviewApplied microbiology and biotechnology2017
Microbial rhamnolipid production: a critical re-evaluation of published data and suggested future publication criteria.
Review in Applied microbiology and biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 100 citations in OpenAlex.
- Purification, Characterization, and Oil-Displacement Performance of Rhamnolipid Biosurfactant Produced byJournal of microbiology and biotechnology · 2026Article
- Variovoracins: new glycolipopeptide biosurfactants identified by surfactant screening and genome mining.RSC advances · 2026Article
- Antimicrobial Proficiency of Amlodipine: Investigating its Impact onIndian journal of microbiology · 2025Article
- Rhamnolipids bio-production and miscellaneous applications towards green technologies: a literature review.PeerJ · 2025Review
- Recombinant Production ofMicroorganisms · 2024Article
- DoE-based medium optimization for improved biosurfactant production withFrontiers in bioengineering and biotechnology · 2024Article
- Characterisation of Milk Microbiota from Subclinical Mastitis and Apparently Healthy Dairy Cattle in Free State Province, South Africa.Veterinary sciences · 2023Article
- New insight into the role of oxygen supply for surfactin production in bench-scale bioreactors using induced surface aeration.Bioprocess and biosystems engineering · 2022Article
- Adaptive evolution of Methylotuvimicrobium alcaliphilum to grow in the presence of rhamnolipids improves fatty acid and rhamnolipid production from CH4.Journal of industrial microbiology & biotechnology · 2022Article
- Overview on Glycosylated Lipids Produced by Bacteria and Fungi: Rhamno-, Sophoro-, Mannosylerythritol and Cellobiose Lipids.Advances in biochemical engineering/biotechnology · 2022Article
- Achieving Commercial Applications for Microbial Biosurfactants.Advances in biochemical engineering/biotechnology · 2022Article
- Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activateProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Biosurfactant based formulation of Pseudomonas guariconensis LE3 with multifarious plant growth promoting traits controls charcoal rot disease in Helianthus annus.World journal of microbiology & biotechnology · 2021Article
- Microbial biosurfactant research: time to improve the rigour in the reporting of synthesis, functional characterization and process development.Microbial biotechnology · 2021Review
- Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery.Microorganisms · 2020Article
- The Variety and Inscrutability of Polar Environments as a Resource of Biotechnologically Relevant Molecules.Microorganisms · 2020Review
- Low-Temperature Biosurfactants from Polar Microbes.Microorganisms · 2020Article
- Characterization of a New Mixture of Mono-Rhamnolipids Produced byMarine drugs · 2020Article
- Exploiting the Natural Diversity of RhlA Acyltransferases for the Synthesis of the Rhamnolipid Precursor 3-(3-Hydroxyalkanoyloxy)Alkanoic Acid.Applied and environmental microbiology · 2020Article
- A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants.Frontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High production cost and potential pathogenicity of Pseudomonas aeruginosa, commonly used for rhamnolipid synthesis, have led to extensive research for safer producing strains and cost-effective production methods. This has resulted in numerous research publications claiming new non-pathogenic producing strains and novel production techniques many of which are unfortunately without proper characterisation of product and/or producing strain/s. Genes responsible for rhamnolipid production have only been confirmed in P. aeruginosa, Burkholderia thailandensis and Burkholderia pseudomallei. Comparing yields in different publications is also generally unreliable especially when different methodologies were used for rhamnolipid quantification. After reviewing the literature in this area, we strongly feel that numerous research outputs have insufficient evidence to support claims of rhamnolipid-producing strains and/or yields. We therefore recommend that standards should be set for reporting new rhamnolipid-producing strains and production yields. These should include (1) molecular and bioinformatic tools to fully characterise new microbial isolates and confirm the presence of the rhamnolipid rhl genes for all bacterial strains, (2) using gravimetric methods to quantify crude yields and (3) use of a calibrated method (high-performance liquid chromatography or ultra-performance liquid chromatography) for absolute quantitative yield determination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.