ArticlePloS one2017
Imported human brucellosis in Belgium: Bio and molecular typing of bacterial isolates, 1996-2015.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Prevalence and detection ofFrontiers in cellular and infection microbiology · 2026Article
- Determination and Characterization of (Novel) Circulating Strains ofPathogens (Basel, Switzerland) · 2025Article
- Molecular epidemiology of brucellosis in Asia: insights from genotyping analyses.Veterinary research communications · 2024Review
- Seroprevalence of Brucellosis in Patients Having Complained of Joint Pain: A Case Control.Cureus · 2023Article
- Identification and molecular characterization of Brucella abortus and Brucella melitensis isolated from milk in cattle in Azerbaijan.BMC veterinary research · 2022Article
- Staggered enforcement of infection control and prevention measures following four consecutive potential laboratory exposures to importedInfection prevention in practice · 2021Article
- A policy analysis of agenda-setting of Brucellosis in Iran using a multiple-stream framework: health policy and historical implications.Journal of preventive medicine and hygiene · 2021Review
- Molecular characteristics of Brucella melitensis isolates from humans in Qinghai Province, China.Infectious diseases of poverty · 2021Article
- Tracking the Origin of Austrian Human Brucellosis Cases Using Whole Genome Sequencing.Frontiers in medicine · 2021Article
- Genetic Characterization ofFrontiers in microbiology · 2021Article
- Molecular investigation of infection sources and transmission chains of brucellosis in Zhejiang, China.Emerging microbes & infections · 2020Article
- Shedding of Brucella melitensis happens through milk macrophages in the murine model of infection.Scientific reports · 2020Article
- Investigation of the molecular characteristics of Brucella isolates from Guangxi Province, China.BMC microbiology · 2019Article
- Whole Genome Sequencing for Tracing Geographical Origin of Imported Cases of Human Brucellosis in Sweden.Microorganisms · 2019Article
- Comparison of PCR-RFLP and PFGE for determining the clonality ofSaudi journal of biological sciences · 2019Article
- Core Genome Multilocus Sequence Typing and Single Nucleotide Polymorphism Analysis in the Epidemiology of Brucella melitensis Infections.Journal of clinical microbiology · 2018Article
- An Overview of Brucellosis in Cattle and Humans, and its Serological and Molecular Diagnosis in Control Strategies.Tropical medicine and infectious disease · 2018Review
- Prevalence and risk factors of brucellosis among veterinary health care professionals.Pathogens and global health · 2017Article
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Authors and funding
11 authors.
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Abstract
objectivesThe aim of this study was to characterize by classical biotyping and Multi-Locus variable number tandem repeats (VNTR) Analysis (MLVA) all Brucella spp. derived from human cases in Belgium from 1996 to 2015. Final goals were to determine the species and biovar, to trace-back on genetic grounds the origin of each strain when patient history and risk factors were missing, and to survey for particular trends at the national level.
methodsA total of 37 Brucella strains, isolated from 37 patients in Belgium, were analyzed by both classical biotyping and MLVA, and the genetic patterns compared to those of human strains isolated worldwide.
resultsClassical biotyping revealed that isolates were mainly Brucella melitensis. Most of them belonged to biovar 3, the most abundant biovar in the Mediterranean region. MLVA confirmed that Brucella melitensis is too diverse in VNTRs to be able to make clusters associated to each biovar, but it allowed retrieving precious epidemiological information. The analysis highlighted the imported nature of the strains from all over the world with a dominant part from the Mediterranean countries. Findings of the MLVA11 testing were in line with the travel history of patients coming from Italy, Turkey, Lebanon and Peru. The analysis was particularly useful because it suggested the geographical origin of the infection for 12/16 patients for whom no case history was available.
conclusionClassical biotyping and MLVA analysis are not exclusive but remain complementary tools for Brucella melitensis strain surveillance. MLVA11 is sufficient for Brucella-free countries such as Belgium to trace the geographical origin of infection, but complete MLVA16 is needed to search for links with endemic areas.
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