ArticlePloS one2017
A novel multicopper oxidase (laccase) from cyanobacteria: Purification, characterization with potential in the decolorization of anthraquinonic dye.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 105 citations in OpenAlex.
- Refinement of Synthetization Parameters for High Laccase-Like Activity of Imidazole-Copper (II) Nitrate Trihydrate Nanozyme Towards an Efficient Biomimetic Nanozyme.Applied biochemistry and biotechnology · 2025Article
- Rapid Colorimetric Detection of Sulfite in Red Wine Using Alginate-Copper Laccase Nanozyme with Smartphone as an Optical Readout.ACS measurement science au · 2025Article
- Biotechnological Applications of Cyanobacteria: Synechocystis and Synechococcus Strains.Advances in biochemical engineering/biotechnology · 2025Review
- Biochemical Characterization of Laccase from Spirulina CPCC-695 and Their Role in Estrone Degradation.The protein journal · 2024Article
- Wheat bran as an efficient agro-process waste for enhanced yellow laccase production by3 Biotech · 2024Article
- Bacterial Nanocellulose/Copper as a Robust Laccase-Mimicking Bionanozyme for Catalytic Oxidation of Phenolic Pollutants.ACS omega · 2023Article
- Optimized culture conditions facilitate the estrone biodegradation ability and laccase activity of Spirulina CPCC-695.Biodegradation · 2023Article
- Eminent Industrial and Biotechnological Applications of Laccases from Bacterial Source: a Current Overview.Applied biochemistry and biotechnology · 2022Review
- Broad thermal spectrum metagenomic laccase with action for dye decolorization and fentin hydroxide treatment.AMB Express · 2022Article
- Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis.Frontiers in bioengineering and biotechnology · 2022Review
- Insights into the biosynthesis pathway of phenolic compounds in microalgae.Computational and structural biotechnology journal · 2022Article
- Toxicity Mitigation of Textile Dye Reactive Blue 4 by Hairy Roots ofBioMed research international · 2022Article
- Enzymatic treatment of phenolic pollutants by a small laccase immobilized on APTES-functionalised magnetic nanoparticles.3 Biotech · 2021Article
- Biodegradation of azo dye-containing wastewater by activated sludge: a critical review.World journal of microbiology & biotechnology · 2021Review
- Structural analysis and biochemical properties of laccase enzymes from two Pediococcus species.Microbial biotechnology · 2021Article
- Article
- Optimisation of the Production and Bleaching Process for a New Laccase from Madurella mycetomatis, Expressed in Pichia pastoris: from Secretion to Yielding Prominent.Molecular biotechnology · 2021Article
- Modelling, docking and simulation analysis of Bisphenol A interaction with laccase from Trichoderma.Bioinformation · 2020Article
- Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes.PloS one · 2020Article
- Optimization of Growth Medium and Fermentation Conditions for the Production of Laccase3 fromMycobiology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel extracellular laccase enzyme produced from Spirulina platensis CFTRI was purified by ultrafiltration, cold acetone precipitation, anion exchange and size exclusion chromatography with 51.5% recovery and 5.8 purification fold. The purified laccase was a monomeric protein with molecular mass of ~66 kDa that was confirmed by zymogram analysis and peptide mass fingerprinting. The optimum pH and temperature of the enzyme activity was found at 3.0 and 30°C using ABTS as substrate but the enzyme was quite stable at high temperature and alkaline pH. The laccase activity was enhanced by Cu+2, Zn+2 and Mn+2. In addition, the dye decolorization potential of purified laccase was much higher in terms of extent as well as time. The purified laccase decolorized (96%) of anthraquinonic dye Reactive blue- 4 within 4 h and its biodegradation studies was monitored by UV visible spectra, FTIR and HPLC which concluded that cyanobacterial laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment.
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