Evidence map›Paper›PMID 28384218›Full record

ArticlePloS one2017

A novel multicopper oxidase (laccase) from cyanobacteria: Purification, characterization with potential in the decolorization of anthraquinonic dye.

Sumbul Afreen, Tooba Naz Shamsi, Mohd Affan Baig, Nadeem Ahmad, Sadaf Fatima, M Irfan Qureshi, Md Imtaiyaz Hassan, Tasneem Fatma

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
15.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 105 citations in OpenAlex.

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  11. Insights into the biosynthesis pathway of phenolic compounds in microalgae.Computational and structural biotechnology journal · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Sumbul AfreenCyanobacterial Biotechnology laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
Tooba Naz ShamsiDepartment of Biotechnology, Jamia Millia Islamia, New Delhi, India.
Mohd Affan BaigDepartment of Biotechnology, Jamia Millia Islamia, New Delhi, India.
Nadeem AhmadCyanobacterial Biotechnology laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
Sadaf FatimaDepartment of Biotechnology, Jamia Millia Islamia, New Delhi, India.
M Irfan QureshiDepartment of Biotechnology, Jamia Millia Islamia, New Delhi, India.
Md Imtaiyaz HassanCenter for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.
Tasneem FatmaCyanobacterial Biotechnology laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.ORCID http://orcid.org/0000-0003-3712-8692
Department of Biotechnology · INJamia Millia Islamia · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel extracellular laccase enzyme produced from Spirulina platensis CFTRI was purified by ultrafiltration, cold acetone precipitation, anion exchange and size exclusion chromatography with 51.5% recovery and 5.8 purification fold. The purified laccase was a monomeric protein with molecular mass of ~66 kDa that was confirmed by zymogram analysis and peptide mass fingerprinting. The optimum pH and temperature of the enzyme activity was found at 3.0 and 30°C using ABTS as substrate but the enzyme was quite stable at high temperature and alkaline pH. The laccase activity was enhanced by Cu+2, Zn+2 and Mn+2. In addition, the dye decolorization potential of purified laccase was much higher in terms of extent as well as time. The purified laccase decolorized (96%) of anthraquinonic dye Reactive blue- 4 within 4 h and its biodegradation studies was monitored by UV visible spectra, FTIR and HPLC which concluded that cyanobacterial laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment.

Indexed as

CatalysisChromatography, GelColoring AgentsCyanobacteriaElectrophoresis, Polyacrylamide GelHydrogen-Ion ConcentrationLaccaseSpectrophotometry, UltravioletSpectroscopy, Fourier Transform InfraredTemperatureColoring AgentsLaccase

Identifiers

PMID28384218
PMCPMC5383238
OpenAlexW2605324800

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.