ArticlePLoS genetics2017
Loss of SLC9A3 decreases CFTR protein and causes obstructed azoospermia in mice.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 46 citations in OpenAlex.
- Soft tissue calcifications in chronic kidney disease-beyond the vasculature.Pflugers Archiv : European journal of physiology · 2025Review
- [Detection of pathogenic gene mutations in thirteen cases of congenital bilateral absence of vas deferens infertility patients].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2024Article
- ARL13B controls male reproductive tract physiology through primary and Motile Cilia.Communications biology · 2024Article
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- Male animal sterilization: history, current practices, and potential methods for replacing castration.Frontiers in veterinary science · 2024Review
- NaInternational journal of molecular sciences · 2023Review
- Tissue-Specific Regulation ofInternational journal of molecular sciences · 2023Review
- A novel role for CFTR interaction with LH and FGF in azoospermia and epididymal maldevelopment caused by cryptorchidism.Basic and clinical andrology · 2022Review
- Congenital Bilateral Absence of the Vas Deferens.Frontiers in genetics · 2022Review
- Congenital absence of the vas deferens with hypospadias or without hypospadias: Phenotypic findings and genetic considerations.Frontiers in genetics · 2022Article
- Sperm preparedness and adaptation to osmotic and pH stressors relate to functional competence of sperm in Bos taurus.Scientific reports · 2021Article
- Genomic, transcriptomic, and protein landscape profile of CFTR and cystic fibrosis.Human genetics · 2021Article
- Genomic Uniqueness of Local Sheep Breeds From Morocco.Frontiers in genetics · 2021Article
- Sperm ion channels and transporters in male fertility and infertility.Nature reviews. Urology · 2021Review
- Genetics of the congenital absence of the vas deferens.Human genetics · 2021Review
- Article
- Analysis of CNVs of CFTR gene in Chinese Han population with CBAVD.Molecular genetics & genomic medicine · 2020Article
- Article
- Mapping the sites of localization of epithelial sodium channel (ENaC) and CFTR in segments of the mammalian epididymis.Journal of molecular histology · 2019Article
- Carbocisteine stimulated an increase in ciliary bend angle via a decrease in [ClPflugers Archiv : European journal of physiology · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene cause cystic fibrosis (CF) and are associated with congenital bilateral absence of the vas deferens (CBAVD), which is the major cause of infertility in male patients with CF. However, most Taiwanese patients with CBAVD do not carry major CFTR mutations. Some patients have a single copy deletion of the solute carrier family 9 isoform 3 (SLC9A3) gene. SLC9A3 is a Na+/H+ exchanger, and depleted Slc9a3 in male mice causes infertility due to the abnormal dilated lumen of the rete testis and efferent ductules. Furthermore, SLC9A3 interacts with CFTR in the pancreatic duct and functions as a genetic modifier of CF. However, SLC9A3 function and its relation to CFTR expression in the male reproductive tract in vivo remain elusive. In the present study, we found that CFTR expression was dramatically decreased in the epididymis and vas deferens of Slc9a3 knockout mice. Adult Slc9a3-/- mice showed not only significantly decreased epididymis and vas deferens weight but also increased testis weight. Furthermore, Slc9a3-/- mice developed obstructive azoospermia because of abnormal abundant secretions and calcification in the lumen of the reproductive tract. Ultrastructural analysis of the epithelium in Slc9a3-/-epididymis and vas deferens displayed disorganized and reduced number of stereocilia and numerous secretory apparatuses. Our data revealed that interdependence between SLC9A3 and CFTR is critical for maintaining a precise microenvironment in the epithelial cytoarchitecture of the male reproductive tract. The Slc9a3-deficient mice with impaired male excurrent ducts in this study provide proof for our clinical findings that some Taiwanese of CBAVD carry SLC9A3 deletion but without major CFTR mutations.
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