Evidence map›Paper›PMID 28382457›Full record

ArticleGastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association2017

Expression and function of Uc.160+, a transcribed ultraconserved region, in gastric cancer.

Ririno Honma, Keisuke Goto, Naoya Sakamoto, Yohei Sekino, Kazuhiro Sentani, Naohide Oue, Wataru Yasui

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 33 citations in OpenAlex.

  1. Review
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  9. Establishment of oxaliplatin-resistant gastric cancer organoids: importance of myoferlin in the acquisition of oxaliplatin resistance.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2021
    Article
  10. Article
  11. The Role of lncRNA in the Development of Tumors, including Breast Cancer.International journal of molecular sciences · 2021
    Review
  12. Article
  13. Article
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  15. Uc.63+ contributes to gastric cancer progression through regulation of NF-kB signaling.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Ririno HonmaDepartment of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8551, Japan.
Keisuke GotoCancer Biology Program, University of Hawaii Cancer Center, 701 Ilalo Street Suite 600, Honolulu, HI, 96813, USA.
Naoya SakamotoDepartment of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8551, Japan.
Yohei SekinoDepartment of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8551, Japan.
Kazuhiro SentaniDepartment of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8551, Japan.
Naohide OueDepartment of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8551, Japan.
Wataru YasuiDepartment of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8551, Japan. wyasui@hiroshima-u.ac.jp.
Hiroshima University · JPUniversity of Hawaii System · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTranscribed ultraconserved regions (T-UCRs) are a novel class of noncoding RNAs that are highly conserved among the orthologous regions in most vertebrates. It has been reported that T-UCRs have distinct signatures in human cancers. We previously discovered the downregulation of T-UCR expression in gastric cancer (GC), indicating that T-UCRs could play an important role in GC biology. Uc.160+, a T-UCR reported to be downregulated in human cancer, has not been examined in GC.

methodsWe analyzed the expression pattern of Uc.160+ in nonneoplastic and tumor tissues of the stomach by using uantitative reverse transcription polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH), specifically focusing on the mechanism of transcriptional regulation and target genes that are regulated by T-UCRs. We also attempted to determine the effect of Uc.160+ expression on biological features of GC cell lines by Western blotting.

resultsOn the basis of the qRT-PCR and ISH results, Uc.160+ expression in adenoma and GC tissues was clearly downregulated compared with that in nonneoplastic mucosa tissues of the stomach. Cancer-specific DNA methylation in the promoter region of Uc.160 was observed by bisulfite genomic DNA sequencing analysis. The effect of DNA methylation on Uc.160+ expression was further confirmed by reporter gene assay. We also revealed that Uc.160+ inhibited the phosphorylation of Akt by regulating phosphatase and tensin homolog (PTEN) expression.

conclusionsThese results indicate that Uc.160+ could possibly have a tumor suppressive role in GC.

Indexed as

AdenocarcinomaAdenomaAgedBase SequenceConserved SequenceDNA MethylationFemaleGene Expression Regulation, NeoplasticHumansMaleMiddle AgedRNA, Long NoncodingStomach NeoplasmsRNA, Long NoncodingDNA methylationGastric cancerNoncoding RNATranscribed ultraconserved region

Identifiers

PMID28382457
OpenAlexW2605189601

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.