ArticleMicrobial cell factories2017
Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 134 citations in OpenAlex.
- Polyhydroxybutyrate (PHB) production from halophiles: a comprehensive review.Bioresources and bioprocessing · 2026Review
- Establishing Halomonas as a chassis for industrial biotechnology: advances in synthetic biology tool development and metabolic engineering strategies.Microbial cell factories · 2025Review
- Developing Quorum Sensing-Based Collaborative Dynamic Control System in Halomonas TD01.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Review
- Microbial Cell Factories in the Bioeconomy Era: From Discovery to Creation.Biodesign research · 2024Review
- Promising non-model microbial cell factories obtained by genome reduction.Frontiers in bioengineering and biotechnology · 2024Review
- Recent Challenges and Trends of Polyhydroxyalkanoate Production by Extremophilic Bacteria Using Renewable Feedstocks.Polymers · 2023Review
- Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species.Biotechnology for biofuels and bioproducts · 2023Article
- Biomimetic natural biomaterials for tissue engineering and regenerative medicine: new biosynthesis methods, recent advances, and emerging applications.Military Medical Research · 2023Review
- Co-production of biofuel, bioplastics and biochemicals during extended fermentation of Halomonas bluephagenesis.Microbial biotechnology · 2023Article
- Novel Production Methods of Polyhydroxyalkanoates and Their Innovative Uses in Biomedicine and Industry.Molecules (Basel, Switzerland) · 2022Review
- Closing the Gap between Bio-Based and Petroleum-Based Plastic through Bioengineering.Microorganisms · 2022Review
- Halomonas spp., as chassis for low-cost production of chemicals.Applied microbiology and biotechnology · 2022Review
- Baseline proteomics characterisation of the emerging host biomanufacturing organism Halomonas bluephagenesis.Scientific data · 2022Article
- Establishment of genetic tools for genomic DNA engineering of Halomonas sp. KM-1, a bacterium with potential for biochemical production.Microbial cell factories · 2022Article
- Control of D-lactic acid content in P(LA-3HB) copolymer in the yeastMetabolic engineering communications · 2022Article
- Reconstruction and optimization of aFrontiers in bioengineering and biotechnology · 2022Article
- Extremophilic BacteriumFrontiers in bioengineering and biotechnology · 2022Article
- CRISPR-Based Approaches for Gene Regulation in Non-Model Bacteria.Frontiers in genome editing · 2022Review
- Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux.Communications biology · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClustered regularly interspaced short palindromic repeats interference (CRISPRi) has provided an efficient approach for targeted gene inhibition. A non-model microorganism Halomonas species TD01 has been developed as a promising industrial producer of polyhydroxyalkanoates (PHA), a family of biodegradable polyesters accumulated by bacteria as a carbon and energy reserve compound. A controllable gene repression system, such as CRISPRi, is needed for Halomonas sp. TD01 to regulate its gene expression levels.
resultsFor the first time CRISPRi was successfully used in Halomonas sp. TD01 to repress expression of ftsZ gene encoding bacterial fission ring formation protein, leading to an elongated cell morphology with typical filamentous shape similar to phenomenon observed with Escherichia coli. CRISPRi was employed to regulate expressions of prpC gene encoding 2-methylcitrate synthase for regulating 3-hydroxyvalerate monomer ratio in PHBV copolymers of 3-hydroxybutyrate (HB) and 3-hydroxyvalerate (HV). Percentages of HV in PHBV copolymers were controllable ranging from less than 1 to 13%. Furthermore, repressions on gltA gene encoding citrate synthase channeled more acetyl-CoA from the tricarboxylic acid (TCA) cycle to poly(3-hydroxybutyrate) (PHB) synthesis. The PHB accumulation by Halomonas sp. TD01 with its gltA gene repressed in various intensities via CRISPRi was increased by approximately 8% compared with the wild type control containing the CRISPRi vector without target.
conclusionsIt has now been confirmed that the CRISPRi system can be applied to Halomonas sp. TD01, a promising industrial strain for production of various PHA and chemicals under open and continuous fermentation process conditions. In details, the CRISPRi system was successfully designed in this study to target genes of ftsZ, prpC and gltA, achieving longer cell sizes, channeling more substrates to PHBV and PHB synthesis, respectively. CRISPRi can be expected to use for more metabolic engineering applications in non-model organisms.
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