Evidence map›Paper›PMID 28381255›Full record

ArticleBMC genomics2017

Male fertility status is associated with DNA methylation signatures in sperm and transcriptomic profiles of bovine preimplantation embryos.

Jenna Kropp, José A Carrillo, Hadjer Namous, Alyssa Daniels, Sana M Salih, Jiuzhou Song, Hasan Khatib

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
14.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 141 citations in OpenAlex.

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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Jenna KroppDepartment of Animal Sciences, University of Wisconsin-Madison, Madison, WI, 53706, USA.
José A CarrilloDepartment of Animal and Avian Sciences, University of Maryland, College Park, MD, 20742, USA.
Hadjer NamousDepartment of Animal Sciences, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Alyssa DanielsDepartment of Animal Sciences, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Sana M SalihDepartment of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, 53792, USA.
Jiuzhou SongDepartment of Animal and Avian Sciences, University of Maryland, College Park, MD, 20742, USA.
Hasan KhatibDepartment of Animal Sciences, University of Wisconsin-Madison, Madison, WI, 53706, USA. hkhatib@wisc.edu.
University of Wisconsin–Madison · USUniversity of Maryland, College Park · USWest Virginia University Hospitals · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundInfertility in dairy cattle is a concern where reduced fertilization rates and high embryonic loss are contributing factors. Studies of the paternal contribution to reproductive performance are limited. However, recent discoveries have shown that, in addition to DNA, sperm delivers transcription factors and epigenetic components that are required for fertilization and proper embryonic development. Hence, characterization of the paternal contribution at the time of fertilization is warranted. We hypothesized that sire fertility is associated with differences in DNA methylation patterns in sperm and that the embryonic transcriptomic profiles are influenced by the fertility status of the bull. Embryos were generated in vitro by fertilization with either a high or low fertility Holstein bull. Blastocysts derived from each high and low fertility bulls were evaluated for morphology, development, and transcriptomic analysis using RNA-Sequencing. Additionally, DNA methylation signatures of sperm from high and low fertility sires were characterized by performing whole-genome DNA methylation binding domain sequencing.

resultsEmbryo morphology and developmental capacity did not differ between embryos generated from either a high or low fertility bull. However, RNA-Sequencing revealed 98 genes to be differentially expressed at a false discovery rate < 1%. A total of 65 genes were upregulated in high fertility bull derived embryos, and 33 genes were upregulated in low fertility derived embryos. Expression of the genes CYCS, EEA1, SLC16A7, MEPCE, and TFB2M was validated in three new pairs of biological replicates of embryos. The role of the differentially expressed gene TFB2M in embryonic development was further assessed through expression knockdown at the zygotic stage, which resulted in decreased development to the blastocyst stage. Assessment of the epigenetic signature of spermatozoa between high and low fertility bulls revealed 76 differentially methylated regions.

conclusionsDespite similar morphology and development to the blastocyst stage, preimplantation embryos derived from high and low fertility bulls displayed significant transcriptomic differences. The relationship between the paternal contribution and the embryonic transcriptome is unclear, although differences in methylated regions were identified which could influence the reprogramming of the early embryo. Further characterization of paternal factors delivered to the oocyte could lead to the identification of biomarkers for better selection of sires to improve reproductive efficiency.

Indexed as

DNA MethylationTranscriptomeAnimalsBlastocystCattleCpG IslandsEmbryonic DevelopmentEpigenomicsFemaleFertilityFertilization in VitroGene Expression ProfilingGene Expression Regulation, DevelopmentalHigh-Throughput Nucleotide SequencingMaleOocytesEmbryonic developmentFertilityIVFMethylationTranscriptome

Identifiers

PMID28381255
PMCPMC5382486
OpenAlexW2605303967

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.