ArticleJournal of neuroscience methods2017
Near-infrared fluorescent protein iRFP713 as a reporter protein for optogenetic vectors, a transgenic Cre-reporter rat, and other neuronal studies.
Article in Journal of neuroscience methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- A systematic guide for identifying transcription factors that directly regulate the expression of a gene of interest.Genome research · 2026Review
- Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia.Scientific reports · 2025Article
- Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia.Research square · 2025Article
- Inversed Effects of Nav1.2 Deficiency at Medial Prefrontal Cortex and Ventral Tegmental Area for Prepulse Inhibition in Acoustic Startle Response.Molecular neurobiology · 2024Article
- Advancing breast cancer diagnosis with a near-infrared fluorescence imaging smart sensor for estrogen/progesterone receptor detection.Scientific reports · 2023Article
- Non-invasive In Vivo Brain Astrogenesis and Astrogliosis Quantification Using a Far-red E2-Crimson Transgenic Reporter Mouse.Molecular neurobiology · 2022Article
- Striatal direct pathway neurons play leading roles in accelerating rotarod motor skill learning.iScience · 2022Article
- Optogenetic manipulation and photoacoustic imaging using a near-infrared transgenic mouse model.Nature communications · 2022Article
- Advances in engineering near-infrared luminescent materials.iScience · 2021Review
- Thermodynamic Implications and Time Evolution of the Interactions of Near-Infrared PbS Quantum Dots with Human Serum Albumin.ACS omega · 2021Article
- Article
- Fast widefield imaging of neuronal structure and function with optical sectioning in vivo.Science advances · 2020Article
- Near-Infrared Fluorescent Proteins: Multiplexing and Optogenetics across Scales.Trends in biotechnology · 2018Review
- Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes in Neuroimaging.Biophysical journal · 2017Article
- Long Evans rat spermatogonial lines are effective germline vectors for transgenic rat production.Transgenic research · 2017Article
Corrections and comments
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Authors and funding
17 authors at 6 institutions in 1 country.
Funding
Abstract
backgroundThe use of genetically-encoded fluorescent reporters is essential for the identification and observation of cells that express transgenic modulatory proteins. Near-infrared (NIR) fluorescent proteins have superior light penetration through biological tissue, but are not yet widely adopted. NEW
methodUsing the near-infrared fluorescent protein, iRFP713, improves the imaging resolution in thick tissue sections or the intact brain due to the reduced light-scattering at the longer, NIR wavelengths used to image the protein. Additionally, iRFP713 can be used to identify transgenic cells without photobleaching other fluorescent reporters or affecting opsin function. We have generated a set of adeno-associated vectors in which iRFP713 has been fused to optogenetic channels, and can be expressed constitutively or Cre-dependently.
resultsiRFP713 is detectable when expressed in neurons both in vitro and in vivo without exogenously supplied chromophore biliverdin. Neuronally-expressed iRFP713 has similar properties to GFP-like fluorescent proteins, including the ability to be translationally fused to channelrhodopsin or halorhodopsin, however, it shows superior photostability compared to EYFP. Furthermore, electrophysiological recordings from iRFP713-labeled cells compared to cells labeled with mCherry suggest that iRFP713 cells are healthier and therefore more stable and reliable in an ex vivo preparation. Lastly, we have generated a transgenic rat that expresses iRFP713 in a Cre-dependent manner.
conclusionsOverall, we have demonstrated that iRFP713 can be used as a reporter in neurons without the use of exogenous biliverdin, with minimal impact on viability and function thereby making it feasible to extend the capabilities for imaging genetically-tagged neurons in slices and in vivo.
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