ArticlePloS one2017
Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis).
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- Genome-Wide Characterization of the wnt Gene Family Reveals aAnimals : an open access journal from MDPI · 2026Article
- High-resolution structure of a fish aquaporin reveals a novel extracellular fold.Life science alliance · 2022Article
- Identification and functional analysis ofFrontiers in physiology · 2022Article
- Cloning and Expression of Four Aquaporin Homologs from the Chinese Black Sleeper (Bostrychus sinensis): The Effects of Salinity Acclimation.Biochemical genetics · 2021Article
- A simple and rapid method for fish sex identification based on recombinase-aided amplification and its use in Cynoglossus semilaevis.Scientific reports · 2021Article
- Seminal Plasma Exosomes: Promising Biomarkers for Identification of Male and Pseudo-Males in Cynoglossus semilaevis.Marine biotechnology (New York, N.Y.) · 2019Article
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
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Abstract
Aquaporin 1 (AQP1) is a member of the transmembrane water channel family of proteins with special structural features, and two AQP1 paralogous genes (aqp1aa and aqp1ab) are reported in teleosts. In the present study, the aqp1aa gene of half-smooth tongue sole (Cynoglossus semilaevis) was cloned and characterized. The full-length cDNA of aqp1aa is 1411 bp with a 786 bp open reading frame encoding a 261-amino acid putative protein with a characteristic structure consisting of 6 membrane-spanning α-helical domains and two highly conserved asparagine-proline-alanine motifs. Real-time quantitative PCR revealed that aqp1aa mRNA is expressed predominantly in the testis of males and pseudo-males, while its expression is low in the ovary and lowest in doublesex and mab-3-related transcription factor 1(DMRT1) knock out fish and triploid males. In situ hybridization indicated that aqp1aa mRNA is expressed mainly in the germ cells of males and pseudo-males, especially in spermatozoa and spermatids. These results suggest that the aqp1aa may play a role in spermatogenesis of C. semilaevis.
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