ArticlePloS one2017
Changes in circulating microRNA levels can be identified as early as day 8 of pregnancy in cattle.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- A Narrative Review of Early Pregnancy Diagnosis Technologies for Livestock: From Conventional to Intelligent Systems.Animals : an open access journal from MDPI · 2026Review
- Placental adaptation to maternal environment in domestic animals.The Journal of reproduction and development · 2026Review
- Technical considerations and review of urinary microRNAs as biomarkers for chronic kidney disease in dogs and cats.Veterinary clinical pathology · 2025Review
- Profiling of blood miRNAomes revealed the potential regulatory role of miRNAs in various lameness phenotypes in feedlot cattle.BMC genomics · 2024Article
- Validation of Selected MicroRNA Transcriptome Data in the Bovine Corpus Luteum during Early Pregnancy by RT-qPCR.Current issues in molecular biology · 2024Article
- Potential of Circulating miRNA Biomarkers and Exosomes for Early Pregnancy Diagnoses in Cattle.Animals : an open access journal from MDPI · 2024Article
- miRNA-protein-metabolite interaction network reveals the regulatory network and players of pregnancy regulation in dairy cows.Frontiers in cell and developmental biology · 2024Review
- Identification of circulatory microRNA based biomarkers for early pregnancy diagnosis in buffalo.Frontiers in cell and developmental biology · 2024Article
- Article
- MicroRNAs in Ruminants and Their Potential Role in Nutrition and Physiology.Veterinary sciences · 2023Review
- Short communication: profiling the expression of Let-7d-5p microRNA in circulating blood of pregnant and nonpregnant cows.Journal of animal science · 2023Article
- Association between blood miR-26a levels following artificial insemination, and pregnancy outcome in dairy cattle.PloS one · 2023Article
- Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep.Frontiers in genetics · 2022Article
- Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth.Journal of ovarian research · 2021Article
- New approaches to diagnose and target reproductive failure in cattle.Animal reproduction · 2020Review
- MiRNAs in the Peri-Implantation Period: Contribution to Embryo-Maternal Communication in Pigs.International journal of molecular sciences · 2020Review
- Review
- MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock.Frontiers in veterinary science · 2020Review
- Farmer and Veterinary Practices and Opinions Related to Fertility Testing and Pregnancy Diagnosis of UK Dairy Cows.Frontiers in veterinary science · 2020Article
- Analysis of circulating-microRNA expression in lactating Holstein cows under summer heat stress.PloS one · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poor reproductive performance remains a major issue in the dairy industry, with low conception rates having a significant impact on milk production through extended calving intervals. A major limiting factor is the lack of reliable methods for early pregnancy diagnosis. Identification of animals within a herd that fail to conceive within 3 weeks after insemination would allow early re-insemination and shorten calving intervals. In a previous study, we found an increase in plasma miR-26a levels in Day 16-pregnant relative to non-pregnant heifers, however changes in miRNA levels that early during pregnancy were very small which likely prevented the identification of robust biomarkers. In this study, we extended our analyses to a wider interval during pregnancy (Days 8 to 60, n = 11 heifers) with the rationale that this may facilitate the identification of additional early pregnancy miRNA biomarkers. Using small RNA sequencing we identified a total of 77 miRNAs that were differentially expressed on Day 60 relative to Day 0 of pregnancy. We selected 14 miRNAs for validation by RT-qPCR and confirmed significant differences in the expression of let-7f, let-7c, miR-30c, miR-101, miR-26a, miR-205 and miR-143 between Days 0 and 60. RT-qPCR profiling throughout Days 0, 8, 16 and 60 of pregnancy showed a distinct increase in circulating levels of miR-26a (3.1-fold, P = 0.046) as early as Day 8 of pregnancy. In summary, in contrast to earlier stages of pregnancy (≤ Day 24), marked differences in the levels of multiple miRNAs can be detected in circulation by Day 60 in cattle. Retrospective analyses showed miR-26a levels to be increased in circulation as early as Day 8, sooner than previously reported in any species, suggesting a biological role for this miRNA in the very early events of pregnancy.
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