ArticlePLoS neglected tropical diseases2017
In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti: An integrated next-generation sequencing approach.
Article in PLoS neglected tropical diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 94 citations in OpenAlex.
- The entomological impact of passive metofluthrin emanators against indoor Aedes aegypti: A randomized field trial.PLoS neglected tropical diseases · 2021Trial
- Resistance to pyrethroids in Aedes aegypti: Insights into transcriptomic response to different insecticide concentrations.PLoS neglected tropical diseases · 2026Article
- Pyrethroid resistance in Aedes aegypti: genetic mechanisms worldwide, and recommendations for effective vector control.Parasites & vectors · 2025Review
- Profiling insecticide resistance phenotypes and genotypes in Aedes aegypti populations across four regions in Puerto Rico.Scientific reports · 2025Article
- Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes inTropical medicine and infectious disease · 2025Article
- Cytochrome P450 and esterase genes associated with permethrin resistance in Aedes aegypti (Linnaeus, 1762).Molecular biology reports · 2025Article
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- The major and minorGigaByte (Hong Kong, China) · 2025Article
- Exploring the Wilderness within: An Integrative Metabolomics and Transcriptomics Study on Near-Wild and ColonizedInsects · 2024Article
- Article
- Potential of MALDI-TOF MS biotyping to detect deltamethrin resistance in the dengue vector Aedes aegypti.PloS one · 2024Article
- Genetic structure and kdr mutations in Aedes aegypti populations along a road crossing the Amazon Forest in Amapá State, Brazil.Scientific reports · 2023Article
- Time-series analysis of transcriptomic changes due to permethrin exposure reveals that Aedes aegypti undergoes detoxification metabolism over 24 h.Scientific reports · 2023Article
- Article
- RNA-Seq-Pop: Exploiting the sequence in RNA sequencing-A Snakemake workflow reveals patterns of insecticide resistance in the malaria vector Anopheles gambiae.Molecular ecology resources · 2023Article
- Strong Positive Selection in Aedes aegypti and the Rapid Evolution of Insecticide Resistance.Molecular biology and evolution · 2023Article
- Optimizing the Application Timing and Dosage of Metarhizium brunneum (Hypocreales: Clavicipitaceae) as a Biological Control Agent of Aedes aegypti (Diptera: Culicidae) Larvae.Journal of medical entomology · 2023Article
- Insecticide resistance levels and associated mechanisms in three Aedes aegypti populations from Venezuela.Memorias do Instituto Oswaldo Cruz · 2023Article
- Rapid Evaporative Ionization Mass Spectrometry (REIMS): a Potential and Rapid Tool for the Identification of Insecticide Resistance in Mosquito Larvae.Journal of insect science (Online) · 2022Article
- Expansive and Diverse Phenotypic Landscape of Field Aedes aegypti (Diptera: Culicidae) Larvae with Differential Susceptibility to Temephos: Beyond Metabolic Detoxification.Journal of medical entomology · 2022Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe capacity of Aedes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. Until alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. Metabolic resistance to pyrethroids is common in Aedes aegypti but the monitoring of the dynamics of resistant alleles is impeded by the lack of robust genomic markers. METHODOLOGY/PRINCIPAL
findingsIn an attempt to identify the genomic bases of metabolic resistance to deltamethrin, multiple resistant and susceptible populations originating from various continents were compared using both RNA-seq and a targeted DNA-seq approach focused on the upstream regions of detoxification genes. Multiple detoxification enzymes were over transcribed in resistant populations, frequently associated with an increase in their gene copy number. Targeted sequencing identified potential promoter variations associated with their over transcription. Non-synonymous variations affecting detoxification enzymes were also identified in resistant populations. CONCLUSION /SIGNIFICANCE: This study not only confirmed the role of gene copy number variations as a frequent cause of the over expression of detoxification enzymes associated with insecticide resistance in Aedes aegypti but also identified novel genomic resistance markers potentially associated with their cis-regulation and modifications of their protein structure conformation. As for gene transcription data, polymorphism patterns were frequently conserved within regions but differed among continents confirming the selection of different resistance factors worldwide. Overall, this study paves the way of the identification of a comprehensive set of genomic markers for monitoring the spatio-temporal dynamics of the variety of insecticide resistance mechanisms in Aedes aegypti.
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