ArticlePlant & cell physiology2017
Light Sheet Fluorescence Microscopy Quantifies Calcium Oscillations in Root Hairs of Arabidopsis thaliana.
Article in Plant & cell physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 60 citations in OpenAlex.
- MAcro Plant Projection Imaging (MAPPI): An open, scalable platform for whole-plant fluorescence real-time imaging.Science advances · 2026Article
- MLO-mediated CaThe New phytologist · 2025Article
- Effect of different colloidal gold nanomaterials on Ganoderma lingzhi fermentation for production of ganoderma polysaccharide and triterpenoid through macroscopic and microscopic investigation.Microbial cell factories · 2025Article
- DIRT/µ: automated extraction of root hair traits using combinatorial optimization.Journal of experimental botany · 2025Article
- A long term time lapse microscopy technique for Arabidopsis roots.Frontiers in plant science · 2025Article
- Full-aperture extended-depth oblique plane microscopy through dynamic remote focusing.Journal of biomedical optics · 2024Article
- Observing ER Dynamics over Long Timescales Using Light Sheet Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity.Nature plants · 2023Article
- Light sheet fluorescence microscopy for the investigation of blood-sucking arthropods dyed via artificial membrane feeding.Parasites & vectors · 2022Article
- The signatures of organellar calcium.Plant physiology · 2021Review
- Plant-environment microscopy tracks interactions ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- Illuminating the hidden world of calcium ions in plants with a universe of indicators.Plant physiology · 2021Review
- Article
- Imaging adult C. elegans live using light-sheet microscopy.Journal of microscopy · 2021Article
- Sensors for the quantification, localization and analysis of the dynamics of plant hormones.The Plant journal : for cell and molecular biology · 2021Review
- Directly imaging the localisation and photosensitization properties of the pan-mTOR inhibitor, AZD2014, in living cancer cells.Journal of photochemistry and photobiology. B, Biology · 2020Article
- Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction.Scientific reports · 2020Article
- The structural bases for agonist diversity in anProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Plant Calcium Signaling in Response to Potassium Deficiency.International journal of molecular sciences · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcium oscillations play a role in the regulation of the development of tip-growing plant cells. Using optical microscopy, calcium oscillations have been observed in a few systems (e.g. pollen tubes, fungal hyphae and algal rhizoids). High-resolution, non-phototoxic and rapid imaging methods are required to study the calcium oscillation in root hairs. We show that light sheet fluorescence microscopy is optimal to image growing root hairs of Arabidopsis thaliana and to follow their oscillatory tip-focused calcium gradient. We describe a protocol for performing live imaging of root hairs in seedlings expressing the cytosol-localized ratiometric calcium indicator Yellow Cameleon 3.6. Using this protocol, we measured the calcium gradient in a large number of root hairs. We characterized their calcium oscillations and correlated them with the rate of hair growth. The method was then used to screen the effect of auxin on the properties of the growing root hairs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.