ArticleScientific reports2017
A Microfluidic Immunostaining System Enables Quality Assured and Standardized Immunohistochemical Biomarker Analysis.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Prognostic biomarkers in oral squamous cell carcinoma: current evidence and future directions.Frontiers in oncology · 2025Review
- The VersaLive platform enables microfluidic mammalian cell culture for versatile applications.Communications biology · 2022Article
- A 3D mixing-based portable magnetic device for fully automatic immunofluorescence staining of γ-H2AX in UVC-irradiated CD4RSC advances · 2020Article
- On-chip immunofluorescence analysis of single cervical cells using an electroactive microwell array with barrier for cervical screening.Biomicrofluidics · 2019Article
- Finger-Actuated Microfluidic Concentration Gradient Generator Compatible with a Microplate.Micromachines · 2019Article
- Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections.Microsystems & nanoengineering · 2019Article
- Combining fluorescence-based image segmentation and automated microfluidics for ultrafast cell-by-cell assessment of biomarkers for HER2-type breast carcinoma.Journal of biomedical optics · 2018Article
- Microfluidic on-chip immunohistochemistry directly from a paraffin-embedded section.Biomicrofluidics · 2018Article
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Authors and funding
5 authors.
Funding
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Abstract
Immunohistochemistry (IHC) plays an important role in biomarker-driven cancer therapy. Although there has been a high demand for standardized and quality assured IHC, it has rarely been achieved due to the complexity of IHC testing and the subjective validation-based process flow of IHC quality control. We present here a microfluidic immunostaining system for the standardization of IHC by creating a microfluidic linearly graded antibody (Ab)-staining device and a reference cell microarray. Unlike conventional efforts, our system deals primarily with the screening of biomarker staining conditions for quantitative quality assurance testing in IHC. We characterized the microfluidic matching of Ab staining intensity using three HER2 Abs produced by different manufacturers. The quality of HER2 Ab was also validated using tissues of breast cancer patients, demonstrating that our system is an efficient and powerful tool for the standardization and quality assurance of IHC.
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