ArticleChemical science2017
Electronic tuning of self-healing fluorophores for live-cell and single-molecule imaging.
Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Allosteric Regulation of Photophysics and Binding in Oxazine-macrocycle Complexes at Single-molecule Resolution.Angewandte Chemie (International ed. in English) · 2026Article
- Single-Molecule Fluorescence Defines a New Dye Chemistry.Chemical & biomedical imaging · 2026Review
- Local GPCR density tips the balance of μ-opioid receptor trafficking.bioRxiv : the preprint server for biology · 2026Article
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- A gentle palette of plasma membrane dyes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Orthogonal Site-Specific Dual Bioconjugation of Aryl and Alkyl Thiols.Journal of the American Chemical Society · 2025Article
- Minimally Invasive DNA-Mediated Photostabilization for Extended Single-Molecule and Super-resolution Imaging.bioRxiv : the preprint server for biology · 2025Article
- Gentle Rhodamines for Live-Cell Fluorescence Microscopy.ACS central science · 2024Article
- Extended voltage imaging in cardiomyocytes with a triplet state quencher-stabilized silicon rhodamine.Bioorganic & medicinal chemistry letters · 2024Article
- Regulation of Absorption and Emission in a Protein/Fluorophore Complex.ACS chemical biology · 2024Article
- Recovering true FRET efficiencies from smFRET investigations requires triplet state mitigation.Nature methods · 2024Article
- Real-time detection of human telomerase DNA synthesis by multiplexed single-molecule FRET.Biophysical journal · 2023Article
- Orange/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Methods for automating the analysis of live-cell single-molecule FRET data.Frontiers in cell and developmental biology · 2023Article
- Leveraging Baird aromaticity for advancement of bioimaging applications.Journal of physical organic chemistry · 2023Article
- Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cholesterol inhibits human voltage-gated proton channel hHv1.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Ultra-photostable DNA FluoroCubes: Mechanism of Photostability and Compatibility with FRET and Dark Quenching.Nano letters · 2022Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Bright, long-lasting organic fluorophores enable a broad range of imaging applications. "Self-healing" fluorophores, in which intra-molecularly linked protective agents quench photo-induced reactive species, exhibit both enhanced photostability and biological compatibility. However, the self-healing strategy has yet to achieve its predicted potential, particularly in the presence of ambient oxygen where live-cell imaging studies must often be performed. To identify key bottlenecks in this technology that can be used to guide further engineering developments, we synthesized a series of Cy5 derivatives linked to the protective agent cyclooctatetraene (COT) and examined the photophysical mechanisms curtailing their performance. The data obtained reveal that the photostability of self-healing fluorophores is limited by reactivity of the COT protective agent. The addition of electron withdrawing substituents to COT reduced its susceptibility to reactions with molecular oxygen and the fluorophore to which it is attached and increased its capacity to participate in triplet energy transfer. Exploiting these insights, we designed and synthesized a suite of modified COT-fluorophores spanning the visible spectrum that exhibited markedly increased intra-molecular photostabilization. Under ambient oxygen conditions, the photostability of Cy3 and Cy5 fluorophore derivatives increased by 3- and 9-fold
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Registered trials
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