ArticleFrontiers in plant science2017
Expression of Foreign Genes Demonstrates the Effectiveness of Pollen-Mediated Transformation in
Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Advances in Tissue Culture-Free Genetic Engineering and Genome Editing of Peanut.Molecular biotechnology · 2026Review
- Genotype-flexible plant genetic transformation: advances and prospects.Frontiers in plant science · 2026Review
- Tissue culture-independent approaches to revolutionizing plant transformation and gene editing.Horticulture research · 2025Article
- Development of high-throughput tissue culture-free plant transformation systems.The Plant journal : for cell and molecular biology · 2025Review
- Modifying the Expression of Cysteine Protease GeneInternational journal of molecular sciences · 2023Article
- SDG102, a H3K36-Methyltransferase-Encoding Gene, Plays Pleiotropic Roles in Growth and Development of Maize (International journal of molecular sciences · 2022Article
- Construction of the SHP-GLOX lignin regulation system and its application in rice straw.Plant methods · 2022Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Application of naturally occurring mechanical forces inPlant signaling & behavior · 2021Review
- Maize transformation: history, progress, and perspectives.Molecular breeding : new strategies in plant improvement · 2021Review
- Genetic Transformation of Apomictic Grasses: Progress and Constraints.Frontiers in plant science · 2021Review
- Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties.Frontiers in plant science · 2021Review
- Article
- Opportunities for Innovation in Genetic Transformation of Forest Trees.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant genetic transformation has arguably been the core of plant improvement in recent decades. Efforts have been made to develop in planta transformation systems due to the limitations present in the tissue-culture-based methods. Herein, we report an improved in planta transformation system, and provide the evidence of reporter gene expression in pollen tube, embryos and stable transgenicity of the plants following pollen-mediated plant transformation with optimized sonication treatment of pollen. The results showed that the aeration at 4°C treatment of pollen grains in sucrose prior to sonication significantly improved the pollen viability leading to improved kernel set and transformation efficiency. Scanning electron microscopy observation revealed that the removal of operculum covering pollen pore by ultrasonication might be one of the reasons for the pollen grains to become competent for transformation. Evidences have shown that the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.